Anti-AHA1抗体[25F2.D9] (ab91259)

概述

  • 产品名称Anti-AHA1抗体[25F2.D9]
    参阅全部 AHA1 一抗
  • 描述
    大鼠单克隆抗体[25F2.D9] to AHA1
  • 经测试应用适用于: WB, IP, ELISA, IHC-P, IHC-Fr, ICC, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Mouse AHA1

  • 阳性对照
    • Rat tissue lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆单克隆
  • 克隆编号25F2.D9
  • 同种型IgG2a
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab91259 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
ELISA
IHC-P
IHC-Fr
ICC
ICC/IF
  • 应用说明ELISA: Use at an assay dependent dilution.
    ICC: Use at an assay dependent dilution.
    ICC/IF: Use at an assay dependent dilution.
    IHC-P: Use at an assay dependent dilution.
    IHC-Fr: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.
    WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 38 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Cochaperone that stimulates HSP90 ATPase activity (By similarity). May affect a step in the endoplasmic reticulum to Golgi trafficking.
    • 组织特异性Expressed in numerous tissues, including brain, heart, skeletal muscle and kidney and, at lower levels, liver and placenta.
    • 序列相似性Belongs to the AHA1 family.
    • 细胞定位Cytoplasm > cytosol. Endoplasmic reticulum. May transiently interact with the endoplasmic reticulum.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Activator of 90 kDa heat shock protein ATPase homolog 1 antibody
      • Activator of heat shock 90kDa protein ATPase homolog 1 antibody
      • AHA 1 antibody
      • AHA1 antibody
      • AHSA 1 antibody
      • Ahsa1 antibody
      • AHSA1_HUMAN antibody
      • C14orf3 antibody
      • HSPC322 antibody
      • p38 antibody
      see all

    Anti-AHA1 antibody [25F2.D9] 图像

    • Anti-AHA1 antibody [25F2.D9] (ab91259) at 1/1000 dilution + Human cell line lysate

      Predicted band size : 38 kDa
    • ab91259 staining AHA1 in HeLa cells by ICC/IF (Immunocytochemistry/immunofluoresence). Cells were fixed with 4% PFA for 20 minutes at room temperature. Samples were incubated with primary antibody (1/100) for 1 hour at room temperature. A Fluoroscein-conjugated goat anti-rat IgG secondary antibody was used.
    • ab91259 used in the Immunoprecipitation of AHA1 from Human Hela cells. The antibody was used at 20µl per 1 ml of cell lysate (50mM Tris (pH 7.2); 150mM NaCl; 1mM MgCl2; 1% TX-100; protease inhibitors); captured on 20µl of protein G beads .

    Anti-AHA1 antibody [25F2.D9] (ab91259)参考文献

    ab91259 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting us. We have 4 anti-AHA1 antibodies: ab91259, ab83036, ab56721 and ab64998. The first 3 antibodies were raised against the full length mouse or human protein, respectively. The sequence homology with these ...

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    Thank you for contacting us. Unfortunately, none of the antibodies you mentioned (ab91259, ab83036, ab13492) have been tested in zebrafish so far. However, we have another HSP90 antibody that was tested in zebrafish: ab13494 (http://w...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"