Anti-AGXT抗体[1F9BC7] (ab110313)

概述

  • 产品名称Anti-AGXT抗体[1F9BC7]
    参阅全部 AGXT 一抗
  • 描述
    小鼠单克隆抗体[1F9BC7] to AGXT
  • 经测试应用适用于: ICC/IF, IP, In-Cell ELISAmore details
  • 种属反应性
    与反应: Rat, Cow, Human
  • 免疫原

    Human liver mitochondria

  • 阳性对照
    • HepG2 cells; Human and Rat liver, Bovine heart and HepG2 cell lysates.
  • 常规说明

    Product was previously marketed under the MitoSciences sub-brand.

性能

应用

Our Abpromise guarantee covers the use of ab110313 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
IP Use at an assay dependent dilution.
In-Cell ELISA Use a concentration of 5 µg/ml.

靶标

  • 组织特异性Liver.
  • 疾病相关Defects in AGXT are the cause of hyperoxaluria primary type 1 (HP1) [MIM:259900]; also known as primary hyperoxaluria type I (PH1) and oxalosis I. HP1 is a rare autosomal recessive inborn error of glyoxylate metabolism characterized by increased excretion of oxalate and glycolate, and the progressive accumulation of insoluble calcium oxalate in the kidney and urinary tract.
  • 序列相似性Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family.
  • 细胞定位Peroxisome. Mitochondrion matrix. Except in some HP1 patients where AGT is found in the mitochondrial matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AGT antibody
    • AGT1 antibody
    • Agxt antibody
    • AGXT1 antibody
    • Alanine glyoxylate aminotransferase antibody
    • Alanine glyoxylate aminotransferase3 antibody
    • Alanine--glyoxylate aminotransferase antibody
    • EC 2.6.1.44 antibody
    • EC 2.6.1.51 antibody
    • Hepatic peroxisomal alanine glyoxylate aminotransferase antibody
    • Hepatic peroxisomal alanine:glyoxylate aminotransferase antibody
    • L alanine glyoxylate aminotransferase 1 antibody
    • MS773 antibody
    • PH1 antibody
    • Serine pyruvate aminotransferase antibody
    • Serine--pyruvate aminotransferase antibody
    • Serine--pyruvate aminotransferase, mitochondrial antibody
    • Serine:pyruvate aminotransferase antibody
    • SPAT antibody
    • SPT antibody
    • SPYA_HUMAN antibody
    • TLH6 antibody
    see all

Anti-AGXT antibody [1F9BC7] 图像

  • Immunocytochemistry analysis using ab110313 at 5 ug/ml staining AGXT in HepG2 cells (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (green).
  • Using MitoSciences' standard immunoprecipitation protocol, ab110313 precipicates with the 43kDa AGXT protein in human liver samples. Very small amounts of AGXT are immunoprecipitated from bovine heart, rat liver or human HepG2 cultured cell lysate. Identity of this protein was confirmed by mass spectrometry. This gel was stained with silver.

Anti-AGXT antibody [1F9BC7] (ab110313)参考文献

ab110313 has not yet been referenced specifically in any publications.

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