概述

  • 产品名称Anti-AGRP抗体
    参阅全部 AGRP 一抗
  • 描述
    兔多克隆抗体to AGRP
  • 经测试应用适用于: IHC-P, ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide from internal sequence residues of Human AGRP (NM_001138.1).

  • 阳性对照
    • Human fetal brain and fetal testis lysates. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HeLa.

性能

应用

Our Abpromise guarantee covers the use of ab113481 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration. PubMed: 24944903
ICC/IF Use a concentration of 10 µg/ml.
WB 1/500 - 1/1000. Predicted molecular weight: 14 kDa.

靶标

  • 功能Plays a role in weight homeostasis. Plays a role in the central control of feeding. Reduces food intake. Inhibits cAMP production mediated by stimulation of melanocortin receptors within the hypothalamus and adrenal gland. Acts primarily on MC3R and MC4R. Has very low activity with MC5R.
  • 组织特异性Expressed primarily in the adrenal gland, subthalamic nucleus, and hypothalamus, with a lower level of expression occurring in testis, lung, and kidney.
  • 疾病相关Genetic variations in AGRP may be a cause of obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
  • 序列相似性Contains 1 agouti domain.
  • 结构域The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.
  • 细胞定位Secreted. Golgi apparatus lumen.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Agouti related neuropeptide antibody
    • Agouti Related Protein Homolog antibody
    • Agouti-related protein antibody
    • Agrp antibody
    • AGRP_HUMAN antibody
    • AGRT antibody
    • ART antibody
    • ASIP2 antibody
    see all

Anti-AGRP antibody 图像

  • ICC/IF image of ab113481 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab113481 at 10µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • All lanes : Anti-AGRP antibody (ab113481) at 1/500 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human fetal testis lysate


    Predicted band size : 14 kDa

Anti-AGRP antibody (ab113481)参考文献

This product has been referenced in:
  • Imbernon M  et al. Hypothalamic KLF4 mediates leptin's effects on food intake via AgRP. Mol Metab 3:441-51 (2014). IHC-P ; Rat . Read more (PubMed: 24944903) »
  • Lopez R  et al. Hyperglycemia enhances the proliferation of non-tumorigenic and malignant mammary epithelial cells through increased leptin/IGF1R signaling and activation of AKT/mTOR. PLoS One 8:e79708 (2013). WB ; Human . Read more (PubMed: 24260287) »

See all 2 Publications for this product

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