• 产品名称Anti-Agrin抗体
    参阅全部 Agrin 一抗
  • 描述
    兔多克隆抗体to Agrin
  • 经测试应用适用于: IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Synthetic peptide from the N terminal residues of Human Agrin protein (NM_198576).

  • 阳性对照
    • Human skeletal muscle.



Our Abpromise guarantee covers the use of ab85174 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/100 - 1/500.


  • 相关性Agrin is an essential extracellular matrix component which promotes clustering of nicotinic acetylcholine receptors (nAChRs) and other proteins during development at the neuromuscular junction. Agrin, MuSK and Rapsyn are all essential components for AChR aggregation, through an unknown mechanism. The C-terminal region of agrin is released into the medium, interacts with receptors on the muscle surface and induces AChR aggregation. The central region contains two O-linked glycosylation sites and a domain homologous to domain III of laminin. The N-terminal region anchors agrin to the extracellular matrix via other basal membrane components. This region also contains a protease inhibitor domain and glycosaminoglycan attachment sites; increasing the predicted MW from 200kDa to ~600kDa.
  • 细胞定位Secreted; extracellular space; extracellular matrix. Note: Synaptic basal lamina at the neuromuscular junction.
  • 数据库链接
  • 别名
    • AGRIN antibody
    • Agrin proteoglycan antibody
    • AGRN antibody
    • FLJ45064 antibody
    • OTTHUMP00000044043 antibody
    see all

Anti-Agrin antibody 图像

  • ab85174 staining Agrin in foetal colon tissue by immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) at a dilution of 1/100.

  • ab85174, at 1/100 dilution, staining Agrin in formalin-fixed, paraffin-embedded human skeletal muscle by Immunohistochemistry.

Anti-Agrin antibody (ab85174)参考文献

ab85174 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Rat embryonic Dorsal Horn Cells in culture)
Permeabilization No
Specification Rat embryonic Dorsal Horn Cells in culture
Fixative 4%PF,0.2% Picric Acid

Ms. Babben Tinner

Verified customer

提交于 Oct 13 2015

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (4-12%)
Sample Human Purified protein (proteoglycan preparation from human milk)
Specification proteoglycan preparation from human milk
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Serena Leone

Verified customer

提交于 Dec 04 2013

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5%
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate Buffer & Tris pH 9.0
Sample Mouse Tissue sections (mouse kidney)
Specification mouse kidney
Permeabilization Yes - 0.2 % Triton X in PBS
Fixative Formaldehyde

Dr. Steffen Rickelt

Verified customer

提交于 Nov 12 2013

The immunogen is a 14 amino acid peptide. It is within this range of amino acids: magrshpgpl rpllpllvva acvlpgaggt cperalerre eeanvvltgt veeilnvdpv.

Thank you for you enquiry regarding ab85174.

As our Abpromise indicates, in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been reported within 6 months of purchase...

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Thank you for contacting Abcam.   I have check with the lab and they have confimred that the immunogen sequence is within the first 1-250aa of the protein. They are unable to provide the actual sequence of the immunogen but I hope that this information...

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