• 产品名称Anti-Agpat2抗体
    参阅全部 Agpat2 一抗
  • 描述
    小鼠多克隆抗体to Agpat2
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Recombinant Fragment
    预测可用于: Mouse, Human
  • 免疫原

    Fusion protein:


    , corresponding to amino acids 49/68 of Mouse Agpat2.

  • 常规说明Produced from outbred CD1 mice

    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.



Our Abpromise guarantee covers the use of ab21816 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 31 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.


  • 功能Converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating an acyl moiety at the sn-2 position of the glycerol backbone.
  • 组织特异性Expressed predominantly in heart and liver.
  • 通路Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 2/3.
  • 疾病相关Defects in AGPAT2 are the cause of congenital generalized lipodystrophy type 1 (CGL1) [MIM:608594]; also known as Berardinelli-Seip congenital lipodystrophy type 1 (BSCL1) or Berardinelli-Seip syndrome. CGL1 is an autosomal recessive disorder characterized by marked paucity of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset of diabetes.
  • 序列相似性Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.
  • 结构域The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 1 acyl sn glycerol 3 phosphate acyltransferase beta antibody
    • 1 acylglycerol 3 phosphate O acyltransferase 2 antibody
    • 1 AGP acyltransferase 2 antibody
    • 1 AGPAT2 antibody
    • 1-acyl-sn-glycerol-3-phosphate acyltransferase beta antibody
    • 1-acylglycerol-3-phosphate O-acyltransferase 2 antibody
    • 1-AGP acyltransferase 2 antibody
    • 1-AGPAT 2 antibody
    • Agpat2 antibody
    • Berardinelli Seip congenital lipodystrophy antibody
    • BSCL antibody
    • BSCL1 antibody
    • EC antibody
    • LPAAB antibody
    • LPAAT beta antibody
    • LPAAT-beta antibody
    • Lysophosphatidic acid acyltransferase beta antibody
    • PLCB_HUMAN antibody
    see all

Anti-Agpat2 antibody 图像

  • All lanes : Anti-Agpat2 antibody (ab21816) at 1/1000 dilution

    Lane 1 : Total protein extract from E. coli with ~50ng to 100ng of a negative control fusion protein with an irrelevant antigen at 20 ug
    Lane 2 : Total protein extract from E. coli with ~50ng to 500ng of the antigen fusion protein at 20 ug

    Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution

    Predicted band size : 31 kDa

Anti-Agpat2 antibody (ab21816)参考文献

ab21816 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (gel 12%)
Sample Mouse Cell lysate - whole cell (Muscle, C2C12)
Specification Muscle, C2C12
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

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提交于 Jan 23 2014