概述

  • 产品名称Anti-Ago1抗体
    参阅全部 Ago1 一抗
  • 描述
    兔多克隆抗体to Ago1
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 1-50 (MEAGPSGAAA GAYLPPLQQV FQAPRRPGIG TVGKPIKLLA NYFEVDIPKI) of Human Ago1 (NP_036331).

  • 阳性对照
    • THP1 cell lysate

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab98056 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 97 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P 1/100.

靶标

  • 功能Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs).
  • 序列相似性Belongs to the argonaute family. Ago subfamily.
    Contains 1 PAZ domain.
    Contains 1 Piwi domain.
  • 细胞定位Cytoplasm > P-body.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AGO1 antibody
    • AGO1_HUMAN antibody
    • Argonaute 1 antibody
    • Argonaute1 antibody
    • eIF 2C 1 antibody
    • eIF-2C 1 antibody
    • eIF2C 1 antibody
    • EIF2C antibody
    • Eif2c1 antibody
    • Eukaryotic translation initiation factor 2C 1 antibody
    • GERP95 antibody
    • Golgi Endoplasmic Reticulum protein 95 kDa antibody
    • hAgo1 antibody
    • Protein argonaute 1 antibody
    • Protein argonaute-1 antibody
    • Putative RNA binding protein Q99 antibody
    • Putative RNA-binding protein Q99 antibody
    • Q99 antibody
    see all

Anti-Ago1 antibody 图像

  • Anti-Ago1 antibody (ab98056) at 1 µg/ml (in 5% skim milk / PBS buffer) + THP1 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 97 kDa
  • ab98056 staining Ago1 in Mouse brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with neutral buffered formalin and blocked with 10% serum for 12 hours at 4°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/100 in TBS, 0.3% Triton X-100 + 10% serum) for 16 hours at 4°C. A biotinylated Goat anti-rabbit IgG (H+L) polyclonal (1/200) was used as the secondary antibody.

    See Abreview

Anti-Ago1 antibody (ab98056)参考文献

This product has been referenced in:
  • Yin Y  et al. Interleukin 7 up-regulates CD95 protein on CD4+ T cells by affecting mRNA alternative splicing: priming for a synergistic effect on HIV-1 reservoir maintenance. J Biol Chem 290:35-45 (2015). WB ; Human . Read more (PubMed: 25411246) »

See 1 Publication for this product

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I would be very happy to raise a discount code for testing ab98056 with ChIP with mouse samples. I would appreciate if you could please provide your customer’s details in order to do it.
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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Mouse Brain Day 25)
Specification Mouse Brain Day 25
Fixative neutral buffered formalin
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Permeabilization Yes - TBS-Tween (0.1%)
Blocking step Serum as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
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提交于 May 28 2012

I am very pleased to hear you would like to accept our offer and test ab98056 in IHC-P in mouse. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview forIHC-P in mouse and include t...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"