概述

  • 产品名称Anti-Ago1抗体
    参阅全部 Ago1 一抗
  • 描述
    兔多克隆抗体to Ago1
  • 经测试应用适用于: IP, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Drosophila melanogaster
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Fruit fly (Drosophila melanogaster) Ago1.

    (Peptide available as ab23538.)

  • 阳性对照
    • This antibody gave a positive signal in Drosophila embryo nuclear extract

性能

应用

Our Abpromise guarantee covers the use of ab5070 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent concentration. PubMed: 18463631
IHC-P Use at an assay dependent concentration. PubMed: 18528333
WB 1/500 - 1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 110 kDa).

靶标

  • 功能Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs).
  • 序列相似性Belongs to the argonaute family. Ago subfamily.
    Contains 1 PAZ domain.
    Contains 1 Piwi domain.
  • 细胞定位Cytoplasm > P-body.
  • Information by UniProt
  • 数据库链接
    • 别名
      • AGO1 antibody
      • AGO1_HUMAN antibody
      • Argonaute 1 antibody
      • Argonaute1 antibody
      • eIF 2C 1 antibody
      • eIF-2C 1 antibody
      • eIF2C 1 antibody
      • EIF2C antibody
      • Eif2c1 antibody
      • Eukaryotic translation initiation factor 2C 1 antibody
      • GERP95 antibody
      • Golgi Endoplasmic Reticulum protein 95 kDa antibody
      • hAgo1 antibody
      • Protein argonaute 1 antibody
      • Protein argonaute-1 antibody
      • Putative RNA binding protein Q99 antibody
      • Putative RNA-binding protein Q99 antibody
      • Q99 antibody
      see all

    Anti-Ago1 antibody 图像

    • Anti-Ago1 antibody (ab5070) at 500 + Drosophila Melanogaster (Fruit Fly) Tissue Lysate at 15 µg

      Secondary
      Sheep anti-rabbit HRP conjugated IgG Polyclonal at 2000

      Performed under reducing conditions.

      Predicted band size : 110 kDa

      This image is courtesy of an anonymous Abreview

      Blot was blocked with 5% Milk for 1 hour at room temperature.

      See Abreview

    • Anti-Ago1 antibody (ab5070) at 1 µg/ml + Drosophila embryo nuclear extract (from melanogaster embryos 0-12Hr) at 10 µg

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size : 110 kDa
      Observed band size : 110 kDa
      Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 30 seconds

    Anti-Ago1 antibody (ab5070)参考文献

    This product has been referenced in:
    • Abe M  et al. Impact of age-associated increase in 2'-O-methylation of miRNAs on aging and neurodegeneration in Drosophila. Genes Dev 28:44-57 (2014). Drosophila melanogaster . Read more (PubMed: 24395246) »
    • Chen N  et al. MicroRNA-410 reduces the expression of vascular endothelial growth factor and inhibits oxygen-induced retinal neovascularization. PLoS One 9:e95665 (2014). ChIP ; Mouse . Read more (PubMed: 24777200) »

    See all 23 Publications for this product

    Product Wall

    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (A549 cells)
    Gel Running Conditions Reduced Denaturing (8%)
    Loading amount 300000 cells
    Specification A549 cells
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    提交于 Jun 13 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunoprecipitation
    Sample Fruit fly (Drosophila melanogaster) Tissue lysate - whole (embryonic cells)
    Total protein in input 1e+006 cells
    Specification embryonic cells
    Immuno-precipitation step Protein A
    Username

    Abcam user community

    Verified customer

    提交于 May 12 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Fruit fly (Drosophila melanogaster) Tissue lysate - whole (many whole cells)
    Loading amount 15 µg
    Specification many whole cells
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    提交于 May 10 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunoprecipitation
    Sample Fruit fly (Drosophila melanogaster) Tissue lysate - whole (Embryo)
    Total protein in input 1 µg
    Specification Embryo
    Immuno-precipitation step Protein G
    Username

    Abcam user community

    Verified customer

    提交于 Nov 23 2010

    Application Western blot
    Sample Schmidtea mediterranea Tissue lysate - whole (many adult somatic stem cells)
    Loading amount 21 µg
    Specification many adult somatic stem cells
    Gel Running Conditions Reduced Denaturing (10%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Username

    Dr. An Zeng

    Verified customer

    提交于 Mar 12 2010

    Thank you for your enquiry. No, to our knowledge this antiserum has not yet been applied using immunoprecipitation. I would be very interested to know how it performs and therefore I am prepared to provide you with a discount code should you wish to...

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    Thank you for your enquiry. Lot# 64529 is no longer available, but the current stock that we do have is from the same rabbit. If you have any additional questions, please contact us again.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"