概述

  • 产品名称
  • 描述
    兔多克隆抗体to Ago1
  • 经测试应用
    适用于: IP, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Drosophila melanogaster
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Fruit fly (Drosophila melanogaster) Ago1.

    (Peptide available as ab23538.)

  • 阳性对照
    • This antibody gave a positive signal in Drosophila embryo nuclear extract

性能

应用

Our Abpromise guarantee covers the use of ab5070 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent concentration. PubMed: 18463631
IHC-P Use at an assay dependent concentration. PubMed: 18528333
WB 1/500 - 1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 110 kDa).

靶标

  • 功能
    Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs).
  • 序列相似性
    Belongs to the argonaute family. Ago subfamily.
    Contains 1 PAZ domain.
    Contains 1 Piwi domain.
  • 细胞定位
    Cytoplasm > P-body.
  • Information by UniProt
  • 数据库链接
    • 别名
      • AGO1 antibody
      • AGO1_HUMAN antibody
      • Argonaute 1 antibody
      • Argonaute 1 RISC catalytic component antibody
      • Argonaute1 antibody
      • eIF 2C 1 antibody
      • eIF-2C 1 antibody
      • eIF2C 1 antibody
      • EIF2C antibody
      • Eif2c1 antibody
      • Eukaryotic translation initiation factor 2C 1 antibody
      • GERP95 antibody
      • Golgi Endoplasmic Reticulum protein 95 kDa antibody
      • hAgo1 antibody
      • Protein argonaute 1 antibody
      • Protein argonaute-1 antibody
      • Putative RNA binding protein Q99 antibody
      • Putative RNA-binding protein Q99 antibody
      • Q99 antibody
      see all

    图片

    • Anti-Ago1 antibody (ab5070) at 500 + Drosophila Melanogaster (Fruit Fly) Tissue Lysate at 15 µg

      Secondary
      Sheep anti-rabbit HRP conjugated IgG Polyclonal at 2000

      Performed under reducing conditions.

      Predicted band size : 110 kDa

      This image is courtesy of an anonymous Abreview

      Blot was blocked with 5% Milk for 1 hour at room temperature.

      See Abreview

    • Anti-Ago1 antibody (ab5070) at 1 µg/ml + Drosophila embryo nuclear extract (from melanogaster embryos 0-12Hr) at 10 µg

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size : 110 kDa
      Observed band size : 110 kDa
      Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 30 seconds

    文献

    This product has been referenced in:
    • Funikov SY  et al. Interplay between RNA interference and heat shock response systems in Drosophila melanogaster. Open Biol 6:N/A (2016). WB ; Drosophila melanogaster . Read more (PubMed: 27805906) »
    • Bailey ST  et al. Loss of estrogen-regulated microRNA expression increases HER2 signaling and is prognostic of poor outcome in luminal breast cancer. Cancer Res 75:436-45 (2015). IP ; Human . Read more (PubMed: 25388283) »

    See all 31 Publications for this product

    客户评价及客户问答

    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (A549 cells)
    Gel Running Conditions
    Reduced Denaturing (8%)
    Loading amount
    300000 cells
    Specification
    A549 cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    提交于 Jun 13 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunoprecipitation
    Sample
    Fruit fly (Drosophila melanogaster) Tissue lysate - whole (embryonic cells)
    Total protein in input
    1e+006 cells
    Specification
    embryonic cells
    Immuno-precipitation step
    Protein A
    Username

    Abcam user community

    Verified customer

    提交于 May 12 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Fruit fly (Drosophila melanogaster) Tissue lysate - whole (many whole cells)
    Loading amount
    15 µg
    Specification
    many whole cells
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    提交于 May 10 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunoprecipitation
    Sample
    Fruit fly (Drosophila melanogaster) Tissue lysate - whole (Embryo)
    Total protein in input
    1 µg
    Specification
    Embryo
    Immuno-precipitation step
    Protein G
    Username

    Abcam user community

    Verified customer

    提交于 Nov 23 2010

    Application
    Western blot
    Sample
    Schmidtea mediterranea Tissue lysate - whole (many adult somatic stem cells)
    Loading amount
    21 µg
    Specification
    many adult somatic stem cells
    Gel Running Conditions
    Reduced Denaturing (10%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Username

    Dr. An Zeng

    Verified customer

    提交于 Mar 12 2010

    Thank you for your enquiry. No, to our knowledge this antiserum has not yet been applied using immunoprecipitation. I would be very interested to know how it performs and therefore I am prepared to provide you with a discount code should you wish to...

    Read More

    Thank you for your enquiry. Lot# 64529 is no longer available, but the current stock that we do have is from the same rabbit. If you have any additional questions, please contact us again.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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