概述

  • 产品名称Anti-Aggrecan抗体
    参阅全部 Aggrecan 一抗
  • 描述
    兔多克隆抗体to Aggrecan
  • 经测试应用适用于: IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Dog
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Rat Aggrecan.

    (Peptide available as ab36860.)

  • 阳性对照
    • Aggrecan G1-IGD-G2 Domains recombinant protein In Western Blot, this antibody gave a positive signal in mouse and rat liver tissue lysate. It also gave a positive result in FFPE human liver sections.

性能

应用

Our Abpromise guarantee covers the use of ab36861 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 250 kDa).Can be blocked with Rat Aggrecan peptide (ab36860).

Abcam recommends using BSA as the blocking agent.

ICC/IF Use at an assay dependent concentration.

靶标

  • 相关性Aggrecan is a member of a family of large, aggregating proteoglycans (also including versican, brevican and neurocan) which is found in articular cartilage. Aggrecan is composed of three major domains: G1, G2, and G3. Between the G1 and G2 domains there is an interglobulin region (IGD). The IGD region is the major site of cleavage by specific proteases like metalloproteinases (MMPs) and aggrecanase. Aggrecan cleavage has been associated with a number of degenerative diseases including rheumatoid arthritis and osteoarthritis. There is evidence that this family of proteoglycans modulates cell adhesion, migration, and axonal outgrowth in the CNS.
  • 细胞定位Secreted; extracellular matrix.
  • 数据库链接
  • 别名
    • ACAN antibody
    • AGC 1 antibody
    • AGC1 antibody
    • AGCAN antibody
    • Aggrecan 1 (chondroitin sulfate proteoglycan 1, large aggregating proteoglycan, antigen identified by monoclonal antibody A0122) antibody
    • Aggrecan 1 antibody
    • Aggrecan core protein antibody
    • Aggrecan proteoglycan antibody
    • Aggrecan structural proteoglycan of cartilage antibody
    • Aggrecan1 antibody
    • ATEGQV antibody
    • Cartilage specific proteoglycan core protein antibody
    • Chondroitin sulfate proteoglycan 1 antibody
    • Chondroitin sulfate proteoglycan 1 large aggregating proteoglycan antigen identified by monoclonal antibody A0122 antibody
    • Chondroitin sulfate proteoglycan core protein 1 antibody
    • CSPG 1 antibody
    • CSPG1 antibody
    • CSPGCP antibody
    • JSCATE antibody
    • Large aggregating proteoglycan antibody
    • mcspg antibody
    • mgsk16 antibody
    • MSK 16 antibody
    • MSK16 antibody
    • SEDK antibody
    see all

Anti-Aggrecan antibody 图像

  • IHC image of Aggrecan staining in rat liver formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab36861, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

  • All lanes : Anti-Aggrecan antibody (ab36861) at 1 µg/ml

    Lane 1 : Liver (Mouse) Tissue Lysate
    Lane 2 : Brain Cortex (Rat)Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 250 kDa
    Observed band size : 250 kDa
    Additional bands at : 45 kDa,50 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes
  • Anti-Aggrecan antibody (ab36861) at 1 µg/ml + Aggrecan G1-IGD-G2 recombinant protein

    Secondary
    Goat polyclonal to Rabbit IgG H&L (HRP) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 250 kDa
    Observed band size : 110 kDa (why is the actual band size different from the predicted?)

    This antibody was tested on Human Aggrecan G1-IGD-G2 Domains recombinant protein which migrates at approximately 120 kDa.

  • IHC image of Aggrecan staining in human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab36861, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • All lanes : Anti-Aggrecan antibody (ab36861) at 1 µg/ml

    Lane 1 : Liver (Mouse) Tissue Lysate
    Lane 2 : Liver (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 250 kDa
    Observed band size : 250 kDa
    Additional bands at : 180 kDa,32 kDa,40 kDa,50 kDa,60 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes

    Abcam recommends using BSA as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

Anti-Aggrecan antibody (ab36861)参考文献

This product has been referenced in:
  • Yang W  et al. Clock gene Bmal1 modulates human cartilage gene expression by crosstalk with Sirt1. Endocrinology N/A:en20152042 (2016). WB ; Human . Read more (PubMed: 27253997) »
  • Liang W  et al. Observing the development of the temporomandibular joint in embryonic and post-natal mice using various staining methods. Exp Ther Med 11:481-489 (2016). IHC-P ; Mouse . Read more (PubMed: 26893634) »

See all 10 Publications for this product

Product Wall

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Hippocampal)
Gel Running Conditions Reduced Denaturing
Loading amount 20 µg
Specification Hippocampal
Blocking step licor blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Jul 29 2016

Abreviews
Application Western blot
Sample Mouse Cell lysate - whole cell (ATDC5 cell)
Gel Running Conditions Reduced Denaturing (7.5%)
Loading amount 25 µg
Treatment 100 nM si RNA for 24h
Specification ATDC5 cell
Blocking step Blocking one (nacalai tesque) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 37°C
Username

Abcam user community

Verified customer

提交于 Nov 11 2015

Application Flow Cytometry
Sample Human Cell (chondrocyte)
Permeabilization Yes - BD Fix Perm Buffer
Gating Strategy Live chondrocytes of median size
Specification chondrocyte
Preparation Cell harvesting/tissue preparation method: fixed and permeabilized cells
Sample buffer: BD Permeabilization Wash Buffer
Fixation BD Fix Perm Buffer
Username

Natalie Woolery

Verified customer

提交于 Aug 03 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid buffer
Sample Mouse Tissue sections (mouse anterior pretectal nucleus midbrain)
Specification mouse anterior pretectal nucleus midbrain
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%
Fixative Paraformaldehyde
Username

Dr. Martin Broadstock

Verified customer

提交于 Feb 26 2015

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (7% gel)
Sample Spermophilus tridecemlineatus Tissue lysate - whole (brain)
Specification brain
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Feb 27 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: RT°C
Antigen retrieval step Heat mediated
Sample Rhesus monkey Tissue sections (Ovarian Tissue)
Specification Ovarian Tissue
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 May 22 2013

Thank you for contacting us.

Let me know if you are interested in our Abtrial offer, we will be happy sending the agreed discount code.

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Muchas gracias por tomarte el tiempo y la molestia de mandarnos el cuestionario relleno.

He revisado el protocolo, y todos los pasos parecen adecuados. Es posible que el lote de anticuerpo recibido fuera defectuoso.

Si me confirmas ...

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It is my pleasure to help.

In this particular group, aggrecan would fluoresce in green while collagen II would be red. We offer other dyes athttp://www.abcam.com/index.html?pageconfig=resource&rid=12956should you wish to change those col...

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Thank you for contacting us.

We presently have only 1 product against mouse aggrecan, ab36861. This is a rabbit polyclonal. In ICC and appropriate secondary would be anti-Rabbit IgG with a fluorescent tag which could be excited by your las...

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