Anti-Aggrecan抗体[6-B-4] (ab3778)

概述

  • 产品名称Anti-Aggrecan抗体[6-B-4]
    参阅全部 Aggrecan 一抗
  • 描述
    小鼠单克隆抗体[6-B-4] to Aggrecan
  • 特异性Recognises the linear amino acid sequence 394EPEEPFTFAPEI406 present in the interglobular domain (IGD) of human and bovine aggrecan (in both intact and matrix protease degraded aggrecan) but not pig and several other animals.
  • 经测试应用适用于: ELISA, IHC-P, IHC-Fr, WB, ICC/IFmore details
  • 种属反应性
    与反应: Cow, Human
  • 免疫原

    Synthetic peptide:

    EPEEPFTFAPEI

    , corresponding to amino acids 413-424 of Human Aggrecan.

  • 常规说明Samples are usually deglycosylated using 0.01 Units Chondroitinase ABC (Sigma), 0.01 Units Keratanase (Seikagaku) and 0.0001 Units Keratanase II (Seikagaku) per 10µg S-GAG of non-deglycosylated aggrecan for optimal epitope recognition in SDS-PAGE and immunohistochemistry – see Little et al. and Caterson et al. Diluted Technomouse culture supernatant.


    This antibody detects aggrecan metabolites (intact or matrix protease-catabolised) in human synovial fluid samples.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液0.2M Tris/HCl pH 7.4, 1-10% foetal calf serum, 0.02% sodium azide
  • 纯度Tissue culture supernatant
  • Primary antibody说明This antibody detects aggrecan metabolites (intact or matrix protease-catabolised) in human synovial fluid samples.
  • 克隆单克隆
  • 克隆编号6-B-4
  • 骨髓瘤x63-Ag8.653
  • 同种型IgG1
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab3778 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
WB 1/100. The antibody will see a large range of aggrecan metabolites/catabolites ranging from greater than 250kD down to 50-60kDa.
ICC/IF Use at an assay dependent concentration. PubMed: 21192818

靶标

  • 相关性Aggrecan is a member of a family of large, aggregating proteoglycans (also including versican, brevican and neurocan) which is found in articular cartilage. Aggrecan is composed of three major domains: G1, G2, and G3. Between the G1 and G2 domains there is an interglobulin region (IGD). The IGD region is the major site of cleavage by specific proteases like metalloproteinases (MMPs) and aggrecanase. Aggrecan cleavage has been associated with a number of degenerative diseases including rheumatoid arthritis and osteoarthritis. There is evidence that this family of proteoglycans modulates cell adhesion, migration, and axonal outgrowth in the CNS.
  • 细胞定位Secreted; extracellular matrix.
  • 数据库链接
  • 别名
    • ACAN antibody
    • AGC 1 antibody
    • AGC1 antibody
    • AGCAN antibody
    • Aggrecan 1 (chondroitin sulfate proteoglycan 1, large aggregating proteoglycan, antigen identified by monoclonal antibody A0122) antibody
    • Aggrecan 1 antibody
    • Aggrecan core protein antibody
    • Aggrecan proteoglycan antibody
    • Aggrecan structural proteoglycan of cartilage antibody
    • Aggrecan1 antibody
    • ATEGQV antibody
    • Cartilage specific proteoglycan core protein antibody
    • Chondroitin sulfate proteoglycan 1 antibody
    • Chondroitin sulfate proteoglycan 1 large aggregating proteoglycan antigen identified by monoclonal antibody A0122 antibody
    • Chondroitin sulfate proteoglycan core protein 1 antibody
    • CSPG 1 antibody
    • CSPG1 antibody
    • CSPGCP antibody
    • JSCATE antibody
    • Large aggregating proteoglycan antibody
    • mcspg antibody
    • mgsk16 antibody
    • MSK 16 antibody
    • MSK16 antibody
    • SEDK antibody
    see all

Anti-Aggrecan antibody [6-B-4] 图像

  • ab3778 at 1/100 staining human articular cartilage by IHC-P. The tissue was formaldehyde fixed and blocked with a serum free protein block. An enzymatic antigen retrieval step was performed and the tissue was then incubated with the antibody for 1 hour. An HRP conjugated goat polyclonal antibody was used as the secondary.

    See Abreview

  • ab3778 used at a 1/50 dilution staining Aggrecan in human umbilical cord matrix mesenchymal stromal cells by Immunocytochemistry/ Immunofluorescence.
    Frozen sections of the 3D constructs (10µm) (n=2) were rehydrated with PBS for 5 minutes, and endogenous peroxidase activity was inhibited using 1% hydrogen peroxide in methanol for 30 minutes. The sections were then blocked in 3% horse serum for 20 minutes and incubated with a primary antibody, ab3778, for 1 hour at a 1/50 dilution. After primary antibody incubation the sections were incubated with a streptavidin linked horse anti-mouse IgG secondary antibody for 30 minutes. After secondary antibody incubation, the sections were incubated with avidin-biotinylated enzyme complex for 30 minutes, and then VIP substrate (purple color) was applied on sections for 4 minutes.

    The image shown is at 4 weeks, 25M.
  • Immunofluorescence analysis of Human articular chondrocytes, staining Aggrecan with ab3778.

    Cells were fixed with paraformaldehyde and blocked with 1% BSA for 1 hour. Cells were incubated with primary antibody overnight at 4°C. An AlexaFluor®488-conjugated rabbit anti-mouse IgG was used as the secondary antibody.

Anti-Aggrecan antibody [6-B-4] (ab3778)参考文献

This product has been referenced in:
  • Zhou X  et al. MicroRNA-99a regulates early chondrogenic differentiation of rat mesenchymal stem cells by targeting the BMPR2 gene. Cell Tissue Res 366:143-53 (2016). Read more (PubMed: 27177866) »
  • Winkler T  et al. Wnt signaling activates Shh signaling in early postnatal intervertebral discs, and re-activates Shh signaling in old discs in the mouse. PLoS One 9:e98444 (2014). IHC-Fr ; Mouse . Read more (PubMed: 24892825) »

See all 14 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.15% · Temperature: RT°C
Antigen retrieval step Heat mediated
Sample Rhesus monkey Tissue sections (Ovarian Section)
Specification Ovarian Section
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 May 22 2013



Sorry but we don't have a standard ELISA procedure for use with this antibody. However I would recommend using an indirect ELISA approach. This is outlined here http://www.abcam.com/index.html?pageconfig=resource&rid=11389

Thank you for contacting us.

Unfortunately the indicated primary antibodies have not been tested with Flow Cytometry, and therefore are not covered by our Abpromise guarantee.

I would instead recommend using a different antibody aga...

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Vielen Dank für Ihren Anruf.



Leider habe ich eine schlechte Nachricht für Sie: Aggrecan hat insgesamt eine Übereinstimmung von 19%, es gibt eine Region zwischen AS 800-850 des menschlichen Proteins, welches eine hoh...

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Thank you for your inquiry.


We have quite a few references for this antibody to be used in ICC/IF and IHC-Fr/IHC-P. You can find them listed here and they are linked to the PubMed IDs.


http://www.abcam.com/Aggrecan-antib...

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Gracias por contactarnos.

Varios marcadores pueden usarse para la detección de condrocitos mediante immunohistoquímica. Solo algunos de ellos se han testado en especie canina, el resto me temo que no se han testado, por lo que s...

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Thank you for contacting us.

For the aggrecan antibody ab3778, I recommend the following two papers, if they are avalable to you, the first of which describes production of this clone and its use for IHC. We do not have access to the journal...

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Thank you very much for your email. Indeed, I have to agree with you that there is a problem. I would like to apologize for this and would like to reassure you that we will investigate this further and take the appropriate actions. The mouse reactivity...

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Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (MEF, R1 ES cells, E14 ES cells)
Specification MEF, R1 ES cells, E14 ES cells
Fixative Paraformaldehyde
Permeabilization Yes - Triton
Username

Abcam user community

Verified customer

提交于 Jun 20 2011

Thank you for your enquiry. Unfortunately, we do not have a Certificate of Analysis for this product. If there is anything else that I can help you with, please do not hesitate to contact me.

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