概述

  • 产品名称Anti-AGFG1抗体
    参阅全部 AGFG1 一抗
  • 描述
    兔多克隆抗体to AGFG1
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rat, Rabbit, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • 免疫原

    Synthetic peptide, corresponding to a region between residues 512 and 562 of Human AGFG1 (NP_004495.2)

  • 阳性对照
    • HeLa or 293T cell lysate.

性能

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应用

Our Abpromise guarantee covers the use of ab86349 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 58 kDa.
IP Use at 2-5 µg/mg of lysate.

靶标

  • 功能Required for vesicle docking or fusion during acrosome biogenesis (By similarity). May play a role in RNA trafficking or localization. In case of infection by HIV-1, acts as a cofactor for viral Rev and promotes movement of Rev-responsive element-containing RNAs from the nuclear periphery to the cytoplasm. This step is essential for HIV-1 replication.
  • 组织特异性Ubiquitously expressed.
  • 序列相似性Contains 1 Arf-GAP domain.
  • 结构域Contains FG repeats. The FG repeat region is required for acting as a cofactor of HIV-1 Rev.
  • 翻译后修饰O-glycosylated.
  • 细胞定位Nucleus. Cytoplasmic vesicle.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AGFG1 antibody
    • AGFG1_HUMAN antibody
    • Arf-GAP domain and FG repeats-containing protein 1 antibody
    • ArfGAP with FG repeats 1 antibody
    • AU045498 antibody
    • C130049H11Rik antibody
    • C85612 antibody
    • D730048C23Rik antibody
    • DKFZp686I15205 antibody
    • HIV 1 Rev binding protein antibody
    • HIV-1 Rev-binding protein antibody
    • HRB antibody
    • MGC116375 antibody
    • MGC116938 antibody
    • MGC116940 antibody
    • Nucleoporin like protein RIP antibody
    • Nucleoporin-like protein RIP antibody
    • RAB antibody
    • Rev interacting protein antibody
    • Rev-interacting protein antibody
    • Rev/Rex activation domain-binding protein antibody
    • RIP antibody
    see all

Anti-AGFG1 antibody 图像

  • All lanes : Anti-AGFG1 antibody (ab86349) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg


    Predicted band size : 58 kDa


    Exposure time : 10 seconds
  • Detection of AGFG1 by Western Blot of Immunprecipitate.
    ab86349 at 1µg/ml staining AGFG1 in HeLa whole cell lysate immunoprecipitated using ab86349 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 3 seconds.

Anti-AGFG1 antibody (ab86349)参考文献

ab86349 has not yet been referenced specifically in any publications.

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