Anti-RAB抗体(ab86349)
Key features and details
- Rabbit polyclonal to RAB
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-RAB抗体
参阅全部 RAB 一抗 -
描述
兔多克隆抗体to RAB -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Rat, Rabbit, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis -
免疫原
Synthetic peptide corresponding to Human RAB aa 512-562.
Database link: NP_004495.2 -
阳性对照
- HeLa or 293T cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab86349于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (2) |
1/2000 - 1/10000. Predicted molecular weight: 58 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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说明 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 58 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
靶标
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功能
Required for vesicle docking or fusion during acrosome biogenesis (By similarity). May play a role in RNA trafficking or localization. In case of infection by HIV-1, acts as a cofactor for viral Rev and promotes movement of Rev-responsive element-containing RNAs from the nuclear periphery to the cytoplasm. This step is essential for HIV-1 replication. -
组织特异性
Ubiquitously expressed. -
序列相似性
Contains 1 Arf-GAP domain. -
结构域
Contains FG repeats. The FG repeat region is required for acting as a cofactor of HIV-1 Rev. -
翻译后修饰
O-glycosylated. -
细胞定位
Nucleus. Cytoplasmic vesicle. - Information by UniProt
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数据库链接
- Entrez Gene: 459990 Chimpanzee
- Entrez Gene: 536149 Cow
- Entrez Gene: 101134648 Gorilla
- Entrez Gene: Human
- Entrez Gene: 3267 Human
- Entrez Gene: 363266 Rat
- Entrez Gene: 708910 Rhesus monkey
- Omim: 600862 Human
see all -
别名
- AGFG1 antibody
- AGFG1_HUMAN antibody
- Arf-GAP domain and FG repeats-containing protein 1 antibody
see all
图片
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All lanes : Anti-RAB antibody (ab86349) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 58 kDa
Exposure time: 10 seconds -
Detection of RAB by Western Blot of Immunprecipitate.
ab86349 at 1µg/ml staining RAB in HeLa whole cell lysate immunoprecipitated using ab86349 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 3 seconds.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab86349 被引用在 2 文献中.
- Fujioka K et al. Wheat Germ Agglutinin (WGA)-SDS-PAGE: A Novel Method for the Detection of O-GlcNAc-modified Proteins by Lectin Affinity Gel Electrophoresis. Bio Protoc 8:e3098 (2018). PubMed: 34532545
- Kubota Y et al. WGA-based lectin affinity gel electrophoresis: A novel method for the detection of O-GlcNAc-modified proteins. PLoS One 12:e0180714 (2017). Human . PubMed: 28686627