概述

  • 产品名称
    Anti-AGE抗体
    参阅全部 AGE 一抗
  • 描述
    兔多克隆抗体to AGE
  • 宿主
    Rabbit
  • 特异性
    ab23722 reacts with Advanced Glycation End Products (AGE), Cross-reacts with BSA and HSA < 1%
  • 经测试应用
    适用于: WB, ICC/IF, ELISA, IHC-Fr, IHC-Pmore details
  • 免疫原

    Advanced Glycation End Products (BSA-AGE and HSA-AGE)

  • 阳性对照
    • Human lens, arteriosclerotic plaques
  • 常规说明


    ab23722 is suitable for the detection of different AGE products in tissues, tissue extracts and body fluids.

应用

Our Abpromise guarantee covers the use of ab23722 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Can be blocked with Native Cow AGE-BSA protein (ab129535).
ICC/IF Use a concentration of 5 µg/ml.
ELISA Use a concentration of 1 µg/ml.
IHC-Fr Use at an assay dependent concentration.
IHC-P 1/10000. PubMed: 19223295

靶标

  • 相关性
    The non enzymatic reaction of reducing carbohydrates with lysine side chains and N terminal amino groups of macromolecules (amino acids, proteins, phospholipids and nucleic acids) is called the Maillard reaction or glycation. The latter products of this process, termed advanced glycation end products (AGEs), adversely affect the functional properties of proteins, lipids and DNA. In long lived tissue proteins, these chemical modifications accumulate with age and may contribute to the pathophysiology of ageing and long term complications of diabetes, atherosclerosis and renal failure.
  • 细胞定位
    Cell Membrane and Secreted
  • 别名
    • Advanced glycation end products antibody
    • AGEs antibody

图片

  • Western blot - Anti-AGE antibody (ab23722)
    Western blot - Anti-AGE antibody (ab23722)This image is courtesy of an Abreview submitted by Josephine Böhme
    All lanes : Anti-AGE antibody (ab23722) at 1/1000 dilution

    Lane 1 : Mouse Brain Lysate(Brain and Hippocampus) at 25 µg
    Lane 2 : Glycated BSA at 15 µg

    Secondary
    All lanes : HRP-conjugated Goat anti-rabbit IgG at 1/800 dilution

    Performed under reducing conditions.


    12% SDS-PAGE gel run using denaturing conditions. Membrane blocked with 5% BSA for 30 minutes at room temperature. Primary antibody diluted in 5% BSA in TBS-T and incubated for 20 hours in a fridge

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGE antibody (ab23722)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGE antibody (ab23722)Image is courtesy of Wang CH et al., PLoS One 9:e90471 (2014), doi: 10.1371/journal.pone.0090471

    ab23722 staining AGE in Rat aoratic tissue sections showing the effects of diabetes and Ator on AGE expression by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formalin. Samples were incubated with primary antibody (1/10000) overnight at 4°C. A secondary antibody Fab fragment was used for 1 hour at room temperature.

    The arrows indicated the sites of antibody staining. NC=normal controls, DM=streptozotocin-induced diabetic rats.

  • Immunocytochemistry/ Immunofluorescence - Anti-AGE antibody (ab23722)
    Immunocytochemistry/ Immunofluorescence - Anti-AGE antibody (ab23722)
    ICC/IF image of ab23722 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab23722, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGE antibody (ab23722)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGE antibody (ab23722)
    ab23722 (1µg/ml) staining AGE in human colon using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of both cell membrane and cytoplasm.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

文献

This product has been referenced in:
  • Kanda A  et al. Advanced glycation endproducts link inflammatory cues to upregulation of galectin-1 in diabetic retinopathy. Sci Rep 7:16168 (2017). Read more (PubMed: 29170525) »
  • de Vos LC  et al. Diverging effects of diabetes mellitus in patients with peripheral artery disease and abdominal aortic aneurysm and the role of advanced glycation end-products: ARTERY study - protocol for a multicentre cross-sectional study. BMJ Open 7:e012584 (2017). Read more (PubMed: 28400456) »

See all 28 Publications for this product

发表研究结果有使用 ab23722？请让我们知道，以便我们可以引用本数据表中的参考文章。

客户评价及客户问答

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27 reviews or Q&A

10
Votes: Highest First

Western blot abreview for Anti-AGE antibody

 Average
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Dermal fibroblasts)
Loading amount
1.8 µg
Specification
Dermal fibroblasts
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
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Username

Dr. Mark Fowler

Verified customer

提交于 Jul 20 2006

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-AGE antibody

 Average
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako Target Antigen Retrival solution EDTA buffer pH9
Permeabilization
No
Specification
Kidney
Blocking step
No blocking step used for 15 minute(s) · Concentration: 0.03 · Temperature: 37°C
Fixative
Paraformaldehyde
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abreview image
Username

Abcam user community

Verified customer

提交于 Jul 10 2017

I would like to know wich types of AGEs the ab23722 recognises in a whole mouse heart extract. Does it recognise only BSA-AGE and HSA-AGE or it recognises the other types of AGEs like CML, pentosidine, imidazolone, pyraline, crossline, AGP, etc.?

We have not tested the cross reactivity with ab23722 and the other types of AGEs that you mentioned. Only BSA-AGE and HSA-AGE have been tested, and the antibody shoes minimial cross reactivity (< 1%) with BSA and HSA alone.

I would like use antiAGE antibody to pig tissue.

Does Anti-AGE antibody (ab23722) work to pig?

This antibody is specific to AGE which is not species specific. Therefore, this antibody can be used in all species including pig.

Does this antibody detect endogenous target or only the immunogens?

This antibody will detect endogenous and artificial AGEs. Mainly in certain diseases as diabetes, arteriosclerosis and Modbus Alzheimer AGE has been detected in various tissues (eye, kidney, artery, brain etc.). In principle all proteins can be affecte...

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Western blot abreview for Anti-AGE antibody

 Good
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain: Cortex and Hippocampus in a mix)
Loading amount
25 µg
Specification
Brain: Cortex and Hippocampus in a mix
Gel Running Conditions
Reduced Denaturing (12 % SDS-PAGE gel)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
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abreview image
Username

Miss. Josephine Böhme

Verified customer

提交于 Apr 09 2013

Inquiry: Hi, We had purchased ab23722 (anti-AGE antibody) awhile ago and I have come across some problems. Following a standard glycation protocol, we can detect the sample with a new anti-CML antibody (Ab125145), but not the Anti-AGE antibody. I am cu...

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The immunogen for ab23722 was prepared as follows:
Under sterile conditions a mixture of BSA and HSA was incubated with D-Glucose in phosphate buffer pH 7.4 at 37°C for some weeks. Low-molecular weight products and D-Glucose were eliminated by...

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We have purchased some antibodies (Anti-RAGE: ab37647 and Anti-AGE: ab23722) from Abcam and require some technical support in regards to one of them. We are looking to perform a Western blot using the anti-AGE antibody and noticed on the dat...

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Thank you for contacting us. The probability of cross-reactivity with BSA is less than 1%.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.


Looking for “realistic, human, available in tissues in clinical pathology lab” positive control for ab62623, could not find for him.

Thank you for contacting us.

Regarding ab62623, Stressed tissue such as Alzheimer's affected neurons. or Human brain pre treated with 10ug proteinase K for 40mins at 37oC are suitable positive controls.

Regarding ab23722, human colon ...

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vielen Dank für Ihre Antwort und ich würde gern Ihr Angebot annehmen.

Desweiteren bestätige ich Ihnen, dass wir ab23722 testen möchten.




vielen Dank, mit freundlichem Gruss

DISCOUNT CODE: ABTRIAL-00000
Ablaufdatum: 12. April 2013
Wert: EURO 410.-

Es freut mich zu hören, dass Sie unser Angebot unseren Antikörper ab23722 in WB zu testen, annehmen möchten. Wie am Telefon besprochen, kö...

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1-10 of 27 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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