概述

  • 产品名称Anti-AGE抗体
    参阅全部 AGE 一抗
  • 描述
    兔多克隆抗体to AGE
  • 特异性ab23722 reacts with Advanced Glycation End Products (AGE), Cross-reacts with BSA and HSA < 1%
  • 经测试应用适用于: WB, ICC/IF, ELISA, IHC-Fr, IHC-Pmore details
  • 免疫原

    Advanced Glycation End Products (BSA-AGE and HSA-AGE)

  • 阳性对照
    • Human lens, arteriosclerotic plaques
  • 常规说明


    ab23722 is suitable for the detection of different AGE products in tissues, tissue extracts and body fluids.

应用

Our Abpromise guarantee covers the use of ab23722 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Can be blocked with Recombinant AGE-BSA protein (ab129535).
ICC/IF Use a concentration of 5 µg/ml.
ELISA Use a concentration of 1 µg/ml.
IHC-Fr Use at an assay dependent concentration.
IHC-P 1/10000. PubMed: 19223295

靶标

  • 相关性The non enzymatic reaction of reducing carbohydrates with lysine side chains and N terminal amino groups of macromolecules (amino acids, proteins, phospholipids and nucleic acids) is called the Maillard reaction or glycation. The latter products of this process, termed advanced glycation end products (AGEs), adversely affect the functional properties of proteins, lipids and DNA. In long lived tissue proteins, these chemical modifications accumulate with age and may contribute to the pathophysiology of ageing and long term complications of diabetes, atherosclerosis and renal failure.
  • 细胞定位Cell Membrane and Secreted
  • 别名
    • Advanced glycation end products antibody
    • AGEs antibody

Anti-AGE antibody 图像

  • All lanes : Anti-AGE antibody (ab23722) at 1/1000 dilution

    Lane 1 : Mouse Brain Lysate(Brain and Hippocampus) at 25 µg
    Lane 2 : Glycated BSA at 15 µg

    Secondary
    HRP-conjugated Goat anti-rabbit IgG at 1/800 dilution

    Performed under reducing conditions.

    This image is courtesy of an Abreview submitted by Josephine Böhme

    12% SDS-PAGE gel run using denaturing conditions. Membrane blocked with 5% BSA for 30 minutes at room temperature. Primary antibody diluted in 5% BSA in TBS-T and incubated for 20 hours in a fridge

    See Abreview

  • ab23722 staining AGE in Rat aoratic tissue sections showing the effects of diabetes and Ator on AGE expression by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formalin. Samples were incubated with primary antibody (1/10000) overnight at 4°C. A secondary antibody Fab fragment was used for 1 hour at room temperature.

    The arrows indicated the sites of antibody staining. NC=normal controls, DM=streptozotocin-induced diabetic rats.

  • ICC/IF image of ab23722 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab23722, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab23722 (1µg/ml) staining AGE in human colon using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of both cell membrane and cytoplasm.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

Anti-AGE antibody (ab23722)参考文献

This product has been referenced in:
  • Rosa CM  et al. Apocynin influence on oxidative stress and cardiac remodeling of spontaneously hypertensive rats with diabetes mellitus. Cardiovasc Diabetol 15:126 (2016). IHC-P ; Rat . Read more (PubMed: 27585437) »
  • Rodriguez-Teja M  et al. AGE-modified basement membrane cooperates with Endo180 to promote epithelial cell invasiveness and decrease prostate cancer survival. J Pathol 235:581-92 (2015). Read more (PubMed: 25408555) »

See all 14 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (Dermal fibroblasts)
Loading amount 1.8 µg
Specification Dermal fibroblasts
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Dr. Mark Fowler

Verified customer

提交于 Jul 20 2006

We have not tested the cross reactivity with ab23722 and the other types of AGEs that you mentioned. Only BSA-AGE and HSA-AGE have been tested, and the antibody shoes minimial cross reactivity (< 1%) with BSA and HSA alone.

This antibody is specific to AGE which is not species specific. Therefore, this antibody can be used in all species including pig.

This antibody will detect endogenous and artificial AGEs. Mainly in certain diseases as diabetes, arteriosclerosis and Modbus Alzheimer AGE has been detected in various tissues (eye, kidney, artery, brain etc.). In principle all proteins can be affecte...

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Application Western blot
Sample Mouse Tissue lysate - whole (Brain: Cortex and Hippocampus in a mix)
Loading amount 25 µg
Specification Brain: Cortex and Hippocampus in a mix
Gel Running Conditions Reduced Denaturing (12 % SDS-PAGE gel)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Miss. Josephine Böhme

Verified customer

提交于 Apr 09 2013

The immunogen for ab23722 was prepared as follows:
Under sterile conditions a mixture of BSA and HSA was incubated with D-Glucose in phosphate buffer pH 7.4 at 37°C for some weeks. Low-molecular weight products and D-Glucose were eliminated by...

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Thank you for contacting us. The probability of cross-reactivity with BSA is less than 1%.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for contacting us.

Regarding ab62623, Stressed tissue such as Alzheimer's affected neurons. or Human brain pre treated with 10ug proteinase K for 40mins at 37oC are suitable positive controls.

Regarding ab23722, human colon ...

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DISCOUNT CODE: ABTRIAL-00000
Ablaufdatum: 12. April 2013
Wert: EURO 410.-

Es freut mich zu hören, dass Sie unser Angebot unseren Antikörper ab23722 in WB zu testen, annehmen möchten. Wie am Telefon besprochen, kö...

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Vielen Dank für Ihre Anfrage.

Ich habe unseren Produktkatalog für sie durchsucht und möchte Ihnen nun ein Angebot machen. Wir haben leider zur Zeit nur einen AGE Antikörper im Angebot und dieser ist unserem Wissen nach noch...

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1-10 of 26 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"