Anti-AGE抗体(ab23722)

Anti-AGE antibody 图像

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  Western blot - Anti-AGE antibody (ab23722)This image is courtesy of an Abreview submitted by Josephine Böhme
  All lanes : Anti-AGE antibody (ab23722) at 1/1000 dilution
  
  Lane 1 : Mouse Brain Lysate(Brain and Hippocampus) at 25 µg
  Lane 2 : Glycated BSA at 15 µg
  
  Secondary
  HRP-conjugated Goat anti-rabbit IgG at 1/800 dilution
  
  Performed under reducing conditions.

  This image is courtesy of an Abreview submitted by Josephine Böhme

  12% SDS-PAGE gel run using denaturing conditions. Membrane blocked with 5% BSA for 30 minutes at room temperature. Primary antibody diluted in 5% BSA in TBS-T and incubated for 20 hours in a fridge

  See Abreview

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGE antibody (ab23722)Image is courtesy of Wang CH et al., PLoS One 9:e90471 (2014), doi: 10.1371/journal.pone.0090471

  ab23722 staining AGE in Rat aoratic tissue sections showing the effects of diabetes and Ator on AGE expression by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formalin. Samples were incubated with primary antibody (1/10000) overnight at 4°C. A secondary antibody Fab fragment was used for 1 hour at room temperature.

  The arrows indicated the sites of antibody staining. NC=normal controls, DM=streptozotocin-induced diabetic rats.

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  Immunocytochemistry/ Immunofluorescence - Anti-AGE antibody (ab23722)
  ICC/IF image of ab23722 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab23722, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  
  
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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGE antibody (ab23722)
  ab23722 (1µg/ml) staining AGE in human colon using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of both cell membrane and cytoplasm.
  Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.