概述

  • 产品名称Anti-AFM抗体
    参阅全部 AFM 一抗
  • 描述
    兔多克隆抗体to AFM
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    A region within synthetic peptide: GQCIINSNKD DRPKDLSLRE GKFTDSENVC QERDADPDTF FAKFTFEYSR, corresponding to amino acids 311-360 of Human AFM

  • 阳性对照
    • HepG2 cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab49139 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 69 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Vitamin E binding protein. May transport vitamin E in body fluids under conditions where the lipoprotein system is not sufficient or across the blood-brain barrier.
  • 序列相似性Belongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • 翻译后修饰N-glycosylated.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AFAM_HUMAN antibody
    • Afamin antibody
    • Afm antibody
    • ALB 2 antibody
    • ALB2 antibody
    • ALBA antibody
    • ALF antibody
    • Alpha albumin antibody
    • Alpha-Alb antibody
    • Alpha-albumin antibody
    see all

Anti-AFM antibody 图像

  • All lanes : Anti-AFM antibody (ab49139) at 0.5 µg/ml

    Lane 1 : Molecular weight marker
    Lane 2 : HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50,000
    Developed using the ECL technique

    Predicted band size : 69 kDa
    Observed band size : 69 kDa
  • IHC image of ab49139 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab49139, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-AFM antibody (ab49139)参考文献

This product has been referenced in:
  • Penno MA  et al. 2D-DIGE analysis of sera from transgenic mouse models reveals novel candidate protein biomarkers for human gastric cancer. J Proteomics : (2012). Read more (PubMed: 22789672) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"