概述

  • 产品名称Anti-AFF4抗体
    参阅全部 AFF4 一抗
  • 描述
    小鼠单克隆抗体to AFF4
  • 经测试应用适用于: IHC-P, WB, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 1-109 of Human AFF4

性能

应用

Our Abpromise guarantee covers the use of ab57077 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 1 µg/ml.
WB Use a concentration of 1 - 5 µg/ml. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 相关性May play a role in transcriptional regulation. Component of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor b (P-TEFb).
  • 细胞定位Nuclear
  • 数据库链接
  • 别名
    • AF4/FMR2 family member 4 antibody
    • AF5Q31 antibody
    • Alf4 antibody
    • ALL1 fused gene from chromosome 5q31 antibody
    • HSPC092 antibody
    • Laf4 antibody
    • Lymphoid nuclear protein related to AF4 like antibody
    • Major CDK9 elongation factor associated protein antibody
    • MCEF antibody
    • MGC107469 antibody
    • RP23-226J16.7 antibody
    see all

Anti-AFF4 antibody 图像

  • AFF4 antibody (ab57077) used in immunohistochemistry at 1ug/ml on formalin fixed and paraffin embedded human endometrium.
  • Western blot against tagged recombinant protein immunogen using ab57077 AFF4 antibody at 1ug/ml. Predicted band size of immunogen is 38 kDa
  • Overlay histogram showing HeLa cells stained with ab57077 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57077, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-AFF4 antibody (ab57077)参考文献

This product has been referenced in:
  • Lu H  et al. AFF1 is a ubiquitous P-TEFb partner to enable Tat extraction of P-TEFb from 7SK snRNP and formation of SECs for HIV transactivation. Proc Natl Acad Sci U S A 111:E15-24 (2014). Read more (PubMed: 24367103) »
  • Liu P  et al. Release of positive transcription elongation factor b (P-TEFb) from 7SK small nuclear ribonucleoprotein (snRNP) activates hexamethylene bisacetamide-inducible protein (HEXIM1) transcription. J Biol Chem 289:9918-25 (2014). WB ; Human . Read more (PubMed: 24515107) »

See all 5 Publications for this product

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