概述

  • 产品名称
  • 描述
    小鼠多克隆抗体to AFAP
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length human AFAP protein (AAH32777.1)

  • 阳性对照
    • AFAP transfected 293T cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab72035 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 81 kDa.

靶标

  • 功能
    Can cross-link actin filaments into both network and bundle structures (By similarity). May modulate changes in actin filament integrity and induce lamellipodia formation. May function as an adapter molecule that links other proteins, such as SRC and PKC to the actin cytoskeleton. Seems to play a role in the development and progression of prostate adenocarcinoma by regulating cell-matrix adhesions and migration in the cancer cells.
  • 组织特异性
    Low expression in normal breast epithelial cell line MCF-10A and in tumorigenic breast cancer cell lines MCF-7, T-47D and ZR75-1. Highly expressed in the invasive breast cancer cell lines MDA-MB-231 and MDA-MB-435. Overexpressed in prostate carcinoma.
  • 序列相似性
    Contains 2 PH domains.
  • 翻译后修饰
    Phosphorylated on tyrosine residues by SRC.
  • 细胞定位
    Cytoplasm > cytoskeleton. Localizes with stress fibers in quiescent cells, concentrated in cell motility structures such as lamellipodia, filopodia and membrane ruffles upon their induction.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 110 kDa actin filament associated protein antibody
    • 110 kDa actin filament-associated protein antibody
    • Actin filament associated protein 1 antibody
    • Actin filament associated protein 110 kDa antibody
    • Actin filament-associated protein 1 antibody
    • AFAP 1 antibody
    • AFAP 110 antibody
    • AFAP antibody
    • AFAP-110 antibody
    • Afap1 antibody
    • AFAP1_HUMAN antibody
    • AFAP110 antibody
    • FLJ56849 antibody
    see all

图片

  • All lanes : Anti-AFAP antibody (ab72035) at 1/500 dilution

    Lane 1 : AFAP transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 81 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)

实验方案

文献

ab72035 has not yet been referenced specifically in any publications.

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