ab121470 shows specific reactivity against Isoform 1 of Human AFAF.
antigen, corresponding to amino acids 35-182 of Human AFAF.
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Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/100 - 1/250.
||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Involved in acrosome biogenesis.
Isoform 1 is highly expressed in testis. Isoform 2 is expressed at low levels in skin and blood.
Cytoplasmic vesicle > secretory vesicle > acrosome membrane.
Information by UniProt
- Acrosome formation-associated factor antibody
- Afaf antibody
- AFAF_HUMAN antibody
Western blot - Anti-AFAF antibody (ab121470)
Lane 1 :
Lanes 2 - 3 : Anti-AFAF antibody (ab121470)
Lane 1 : Marker
Lane 2 : HEK293T cell lysate - vector only transfected
Lane 3 : HEK293T cell lysate - overexpressing AFAF with a C-terminal myc-DDK tag (~3.1kDa)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AFAF antibody (ab121470)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling AFAF with ab121470. Staining shows strong cytoplasmic positivity in spermatids.
has not yet been referenced specifically in any publications.
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