概述

  • 产品名称Anti-AFAF抗体
    参阅全部 AFAF 一抗
  • 描述
    兔多克隆抗体to AFAF
  • 经测试应用适用于: IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen: SCESQYSVNP ELATMSYFHP SEGVSDTSFS KSAESSTFLG TTSSDMRRSG TRTSESKIMT DIISIGSDNE MHENDESVTR, corresponding to amino acids 215-294 of Human AFAF

  • 阳性对照
    • Human testis tissue

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab121461 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

靶标

  • 功能Involved in acrosome biogenesis.
  • 组织特异性Isoform 1 is highly expressed in testis. Isoform 2 is expressed at low levels in skin and blood.
  • 细胞定位Cytoplasmic vesicle > secretory vesicle > acrosome membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Acrosome formation-associated factor antibody
    • Afaf antibody
    • AFAF_HUMAN antibody
    • C9orf11 antibody
    • chromosome 9 open reading frame 11 antibody
    • equatorin antibody
    see all

Anti-AFAF antibody 图像

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus but not nucleoli and vesicles. Recommended concentration of ab121461 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121461 at a 1/300 dilution staining AFAF in Human testis tissue (paraffin embedded) by immunohistochemistry.

Anti-AFAF antibody (ab121461)参考文献

ab121461 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab121461.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"