This fast track antibody is not yet fully characterized. It is subject to these terms and conditions

概述

  • 产品名称Anti-AE2抗体
    参阅全部 AE2 一抗
  • 描述
    兔多克隆抗体to AE2

    This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »

  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Horse, Guinea pig, Cow, Orangutan
  • 免疫原

    Synthetic peptide derived from within residues 200 - 300 of Human AE2.

    (Peptide available as ab42686.)

  • 阳性对照
    • This antibody gave a positive signal when tested on MCF7 cells in IF

性能

应用

Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration. PubMed: 21705333

靶标

Anti-AE2 antibody 图像

This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

  • ICC/IF image of ab42687 stained MCF7 cells. The cells were 100% methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab42687, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in Hek293 cells fixed in 100% methanol at 5ug/ml. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.

Anti-AE2 antibody (ab42687)参考文献

This product has been referenced in:
  • Kao L  et al. Severe Neurologic Impairment in Mice with Targeted Disruption of the Electrogenic Sodium Bicarbonate Cotransporter NBCe2 (Slc4a5 Gene). J Biol Chem 286:32563-74 (2011). IHC-FoFr ; Mouse . Read more (PubMed: 21705333) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Lung, Liver)
Loading amount 25 µg
Specification Lung, Liver
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jul 15 2009

Application Western blot
Sample Human Cell lysate - whole cell (HeLa, U2OS, NIH-3T3, MEF)
Loading amount 20 µg
Specification HeLa, U2OS, NIH-3T3, MEF
Gel Running Conditions Reduced Denaturing (7.5% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Mr. Antibody Solutions

Verified customer

提交于 Jan 27 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"