Anti-ADRM1/ARM-1抗体(ab91567)
Key features and details
- Rabbit polyclonal to ADRM1/ARM-1
- Suitable for: IP, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-ADRM1/ARM-1抗体
参阅全部 ADRM1/ARM-1 一抗 -
描述
兔多克隆抗体to ADRM1/ARM-1 -
宿主
Rabbit -
经测试应用
适用于: IP, WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Chimpanzee, Rhesus monkey, Orangutan -
免疫原
Synthetic peptide corresponding to Human ADRM1/ARM-1 aa 357-407.
Database link: NP_008933.2 -
阳性对照
- HeLa and 293T whole cell lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab91567 was affinity purified using an epitope specific to ADRM1/ARM-1 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab91567于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IP |
Use at 5-15 µg/mg of lysate.
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WB |
1/2000 - 1/10000. Predicted molecular weight: 42 kDa.
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IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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IP
Use at 5-15 µg/mg of lysate. |
WB
1/2000 - 1/10000. Predicted molecular weight: 42 kDa. |
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Functions as a proteasomal ubiquitin receptor. Recruits the deubiquitinating enzyme UCHL5 at the 26S proteasome and promotes its activity. -
序列相似性
Belongs to the ADRM1 family.
Contains 1 PH domain. -
结构域
The PH domain mediates interactions with PSMD1 and ubiquitin. Preferential binding to the proximal subunit of K48-linked diubiquitin allows UCHL5 access to the distal subunit. -
细胞定位
Cytoplasm. Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 11047 Human
- Omim: 610650 Human
- SwissProt: Q16186 Human
- Unigene: 90107 Human
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别名
- 110 kDa cell membrane glycoprotein antibody
- adhesion regulating molecule 1 antibody
- Adhesion-regulating molecule 1 antibody
see all
图片
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All lanes : Anti-ADRM1/ARM-1 antibody (ab91567) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 42 kDa
Exposure time: 3 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling ADRM1/ARM-1 with ab91567 at 1/1000 (1µg/ml). Detection: DAB.
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Detection of ADRM1/ARM-1 by Western Blot of Immunprecipitate.
ab91567, at 1 µg/ml, staining ADRM1/ARM-1 in HeLa whole cell lysate immunoprecipitated using ab91567 at 10 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab91567 被引用在 2 文献中.
- Huang Y & Ratovitski EA Phosphorylated TP63 induces transcription of RPN13, leading to NOS2 protein degradation. J Biol Chem 285:41422-31 (2010). WB ; Human . PubMed: 20959455
- Huang Y & Ratovitski EA Phospho-?Np63a/Rpn13-dependent regulation of LKB1 degradation modulates autophagy in cancer cells. Aging (Albany NY) 2:959-68 (2010). PubMed: 21191146