Anti-Adrenomedullin抗体[HTA171/E8] (ab18092)
- 数据表
- 具体的参考文献 (3)
- 实验方案
概述
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产品名称Anti-Adrenomedullin抗体[HTA171/E8]
参阅全部 Adrenomedullin 一抗 -
描述小鼠单克隆抗体[HTA171/E8] to Adrenomedullin
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宿主Mouse
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经测试应用适用于: Flow Cyt, IHC-P, IHC-Frmore details
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种属反应性与反应: Human
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免疫原
Chemically synthesized full length adrenomedullin.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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存储溶液Preservative: None
Constituents: PBS -
Concentration information loading... -
纯度Purified IgM
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克隆单克隆
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克隆编号HTA171/E8
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同种型IgM
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研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
Our Abpromise guarantee covers the use of ab18092 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt | Use 1µg for 106 cells. ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.
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| IHC-P | 1/50. PubMed: 18445660 | |
| IHC-Fr | Use at an assay dependent concentration. |
靶标
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功能AM and PAMP are potent hypotensive and vasodilatator agents. Numerous actions have been reported most related to the physiologic control of fluid and electrolyte homeostasis. In the kidney, am is diuretic and natriuretic, and both am and pamp inhibit aldosterone secretion by direct adrenal actions. In pituitary gland, both peptides at physiologically relevant doses inhibit basal ACTH secretion. Both peptides appear to act in brain and pituitary gland to facilitate the loss of plasma volume, actions which complement their hypotensive effects in blood vessels.
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组织特异性Highest levels found in pheochromocytoma and adrenal medulla. Also found in lung, ventricle and kidney tissues.
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序列相似性Belongs to the adrenomedullin family.
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细胞定位Secreted.
- Information by UniProt
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数据库链接
- Entrez Gene: 133 Human
- Omim: 103275 Human
- SwissProt: P35318 Human
- Unigene: 441047 Human
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别名
- Adm antibody
- ADM precursor antibody
- ADML_HUMAN antibody
see all
图片
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![Flow Cytometry - Anti-Adrenomedullin antibody [HTA171/E8] (ab18092)](http://www.abcam.cn/ps/datasheet/images/18/ab18092/Adrenomedullin-Primary-antibodies-ab18092-1.jpg)
Flow Cytometry - Anti-Adrenomedullin antibody [HTA171/E8] (ab18092)Overlay histogram showing PC12 cells stained with ab18092 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab18092, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgM (mu chain) (ab97007) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
文献
This product has been referenced in:
- Kraus DM et al. Cigarette Smoke-Induced Placental Adrenomedullin Expression and Trophoblast Cell Invasion. Reprod Sci N/A:N/A (2013). Read more (PubMed: 23653390) »
- Petalidis LP et al. Improved grading and survival prediction of human astrocytic brain tumors by artificial neural network analysis of gene expression microarray data. Mol Cancer Ther : (2008). IHC-P ; Human . Read more (PubMed: 18445660) »
客户评价及客户问答
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