Anti-Adrenomedullin抗体[HTA171/E8] (ab18092)

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概述

  • 产品名称
    Anti-Adrenomedullin抗体[HTA171/E8]
    参阅全部 Adrenomedullin 一抗
  • 描述
    小鼠单克隆抗体[HTA171/E8] to Adrenomedullin
  • 经测试应用
    适用于: Flow Cyt, IHC-P, IHC-Frmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Chemically synthesized full length adrenomedullin.

性能

应用

Our Abpromise guarantee covers the use of ab18092 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1µg for 106 cells. ab91545-Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.
IHC-P 1/50. PubMed: 18445660
IHC-Fr Use at an assay dependent concentration.

靶标

  • 功能
    AM and PAMP are potent hypotensive and vasodilatator agents. Numerous actions have been reported most related to the physiologic control of fluid and electrolyte homeostasis. In the kidney, am is diuretic and natriuretic, and both am and pamp inhibit aldosterone secretion by direct adrenal actions. In pituitary gland, both peptides at physiologically relevant doses inhibit basal ACTH secretion. Both peptides appear to act in brain and pituitary gland to facilitate the loss of plasma volume, actions which complement their hypotensive effects in blood vessels.
  • 组织特异性
    Highest levels found in pheochromocytoma and adrenal medulla. Also found in lung, ventricle and kidney tissues.
  • 序列相似性
    Belongs to the adrenomedullin family.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
    • 别名
      • Adm antibody
      • ADM precursor antibody
      • ADML_HUMAN antibody
      • AM antibody
      • PAMP antibody
      • Preproadrenomedullin antibody
      • Proadrenomedullin N-20 terminal peptide antibody
      • Proadrenomedullin N20 terminal peptide antibody
      • ProAM N terminal 20 peptide antibody
      • ProAM N-terminal 20 peptide antibody
      • ProAM N20 antibody
      • ProAM-N20 antibody
      • ProAMN20 antibody
      see all

    Anti-Adrenomedullin antibody [HTA171/E8] 图像

    • Flow Cytometry - Anti-Adrenomedullin antibody [HTA171/E8] (ab18092)
      Flow Cytometry - Anti-Adrenomedullin antibody [HTA171/E8] (ab18092)
      Overlay histogram showing PC12 cells stained with ab18092 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab18092, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgM (mu chain) (ab97007) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    Anti-Adrenomedullin antibody [HTA171/E8] (ab18092)参考文献

    This product has been referenced in:
    • Kraus DM  et al. Cigarette Smoke-Induced Placental Adrenomedullin Expression and Trophoblast Cell Invasion. Reprod Sci N/A:N/A (2013). Read more (PubMed: 23653390) »
    • Petalidis LP  et al. Improved grading and survival prediction of human astrocytic brain tumors by artificial neural network analysis of gene expression microarray data. Mol Cancer Ther : (2008). IHC-P ; Human . Read more (PubMed: 18445660) »

    See all 3 Publications for this product

    发表研究结果有使用 ab18092？请让我们知道，以便我们可以引用本数据表中的参考文章。

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    1 Abreview or Q&A

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    I have recentny purchasesd this antibody but I am difficulty detecting any protein in my samples by a western. I have also purchased chemically synthesised adrenomedullin from Bachem and am unable to detect a band for this with a western either. Here i...

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    Thank you for contacting us for technical support with ab18092. This antibody has not been tested in western blotting, only in immunohistochemistry, and your protocol looks very good so this suggest to me that the antibody may only recognise the native...

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