• 产品名称
    参阅全部 Adrenomedullin 一抗
  • 描述
    兔多克隆抗体to Adrenomedullin
  • 宿主
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rat, Horse, Pig不与反应: Mouse
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Adrenomedullin.

    (Peptide available as ab69116.)

  • 阳性对照
    • Thia antibody gave a positive signal in the following Human Lysates: Breast Tissue MCF7 Whole Cell DU 145 Whole Cell MDA-MB-361 Whole Cell This antibody gave a positive result in IHC in the following FFPE tissue: Human placenta.



Our Abpromise guarantee covers the use of ab69117 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 20 kDa).Can be blocked with Human Adrenomedullin peptide (ab69116).
IHC-P Use a concentration of 5 µg/ml.


  • 功能
    AM and PAMP are potent hypotensive and vasodilatator agents. Numerous actions have been reported most related to the physiologic control of fluid and electrolyte homeostasis. In the kidney, am is diuretic and natriuretic, and both am and pamp inhibit aldosterone secretion by direct adrenal actions. In pituitary gland, both peptides at physiologically relevant doses inhibit basal ACTH secretion. Both peptides appear to act in brain and pituitary gland to facilitate the loss of plasma volume, actions which complement their hypotensive effects in blood vessels.
  • 组织特异性
    Highest levels found in pheochromocytoma and adrenal medulla. Also found in lung, ventricle and kidney tissues.
  • 序列相似性
    Belongs to the adrenomedullin family.
  • 细胞定位
  • Information by UniProt
  • 数据库链接
  • 别名
    • Adm antibody
    • ADM precursor antibody
    • ADML_HUMAN antibody
    • AM antibody
    • PAMP antibody
    • Preproadrenomedullin antibody
    • Proadrenomedullin N-20 terminal peptide antibody
    • Proadrenomedullin N20 terminal peptide antibody
    • ProAM N terminal 20 peptide antibody
    • ProAM N-terminal 20 peptide antibody
    • ProAM N20 antibody
    • ProAM-N20 antibody
    • ProAMN20 antibody
    see all


  • All lanes : Anti-Adrenomedullin antibody (ab69117) at 1 µg/ml

    Lane 1 : Human breast tissue lysate - total protein (ab30090)
    Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 3 : DU 145 (Human prostate carcinoma cell line) Whole Cell Lysate
    Lane 4 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 20 kDa
    Observed band size: 22 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 170 kDa, 70 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 8 minutes
  • IHC image of Adrenomedullin staining in Human placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab69117, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.


This product has been referenced in:
  • Maia LL  et al. JMJD1A, H3K9me1, H3K9me2 and ADM expression as prognostic markers in oral and oropharyngeal squamous cell carcinoma. PLoS One 13:e0194884 (2018). Read more (PubMed: 29590186) »
  • Albertin G  et al. Urotensin-II-stimulated expression of pro-angiogenic factors in human vascular endothelial cells. Regul Pept : (2011). WB ; Human . Read more (PubMed: 21871928) »

See all 2 Publications for this product


We have not carried out a peptide blocking study for this product. This has been characterised using WB against MCF7, DU145 and MDA MB 361, and IHC against Human plancenta.

We guarantee the antibody to be specific for adrenomedullin for 1...

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Western blot
Loading amount
30000 cells
Gel Running Conditions
Reduced Denaturing (11)
Mouse Cell lysate - whole cell (Mouse embryonic fibroblast (MEF))
Mouse embryonic fibroblast (MEF)
0.5% oxygen for 6hrs
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

提交于 Nov 05 2013

Western blot
Loading amount
30000 cells
Gel Running Conditions
Reduced Denaturing (11%)
Human Cell lysate - whole cell (MCF-7 cells (human breast adenocarcinoma cell line)
MCF-7 cells (human breast adenocarcinoma cell line
0.5% oxygen for 16hrs
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

提交于 Nov 05 2013

I can confirm that this antibody has been tested for both applications Western blot (WB) and IHC on formalin-fixed paraffin-embedded tissue sections (IHC-P) using human samples. The cross-reactivity with other species has not been determined yet, thoug...

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