ADP Assay试剂盒(Colorimetric/Fluorometric) (ab83359)

概述

  • 产品名称ADP Assay试剂盒(Colorimetric/Fluorometric)
  • 测试
    100 x 1 assay
  • 样品类型
    Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media
  • 检测类型Quantitative
  • 灵敏度
    > 1 µM
  • 检测时间
    1h 00m
  • 种属反应性
    与反应: Human
    预测可用于: all Mammals
  • 产品概述

    ADP Assay Kit (Colorimetric/Fluorometric) (ab83359) provides a convenient colorimetric and fluorometric method to measure ADP level. In the assay, ADP is converted to ATP and pyruvate. The generated pyruvate can be quantified by colorimetric (ODmax = 570 nm) or fluorometric method (Ex/Em 535/587 nm). Conventionally, ADP levels are measured by luciferase/luciferin mediated assays after ADP is converted to ATP. However, the luciferase system is unstable and luminescence equipment is not generally available in most laboratories. In comparison, this assay is simple, sensitive, stable and high-throughput adaptable and can be used with conventional microplate readers. The assay can detect as low as 1 μM ADP in biological samples.


    Visit our FAQs page for tips and troubleshooting.

  • 说明

    ADP is a product of ATP de-phosphorylation and it can be rephosphorylated to ATP. De-phosphorylation and re-phosphorylation occur via various phosphatases, phosphorylases and kinases. ADP is stored in platelets and can be released to interact with a variety of purinergic receptors. ADP levels regulate several enzymes involved in intermediary metabolism. ADP conversion to ATP primarily occurs within the mitochondrion and chloroplast although several such processes occur in the cytoplasm.

  • 经测试应用适用于: Functional Studiesmore details

性能

  • 相关性ADP (Adenosine diphosphate) is a product of ATP dephosphorylation and it can be rephosphorylated to ATP. Dephosphorylation and rephosphorylation occur via various phosphatases, phosphorylases and kinases. ADP is stored in platelets and can be released to interact with a variety of purinergic receptors. ADP levels regulate several enzymes involved in intermediary metabolism. ADP conversion to ATP primarily occurs within the mitochondrion and chloroplast although several such processes occur in the cytoplasm.
  • 别名
    • Adenosine diphosphate

应用

Our Abpromise guarantee covers the use of ab83359 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent dilution.

ADP Assay Kit (Colorimetric/Fluorometric) 图像

  • ADP levels measured fluorometrically in cell culture supernatants (background signal subtracted, mean of duplicates; +/- SD).

  • ADP levels measured fluorometrically in cell lysates (background signal subtracted, mean of duplicates; +/- SD).

  • APD levels colorimetrically measured in biological fluids (rat plasma and serum, human saliva); background signal subtracted, mean of duplicates; +/- SD.

  • Colorimetric and Fluorometric examples of ADP Standard curve.

实验方案

ADP Assay Kit (Colorimetric/Fluorometric) (ab83359)参考文献

This product has been referenced in:
  • Mayeur S  et al. Maternal calorie restriction modulates placental mitochondrial biogenesis and bioenergetic efficiency: putative involvement in fetoplacental growth defects in rats. Am J Physiol Endocrinol Metab 304:E14-22 (2013). Functional Studies . Read more (PubMed: 23092912) »

See 1 Publication for this product

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Thank you for your inquiry.

I would recommend the spin column filters (ab93349) over the PCA treatment for deproteinization. Theoretically the PCA treatment should be fine, but since we have not tested the outcome of this assay with such trea...

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Thank you for contacting us.

Although the laboratory has not tested in the conditions you describe, using a reaction with an enzyme, they believe their is no compatibility issue and that it should work with some optimization. Same goes for t...

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Merci pour votre appel de vendredi.

Le laboratoire m'a pu confirmer que vous pouvez procéder sans lescolonnes:

Pour le kit ADP ab83359, les échantillons lysés dans le"ADP assay buffer" peuventêtr...

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Unfortunately we do not give out our buffer components or compositions. Sorry about that.

Yes, it is very likely that the ATP in your buffer is giving the background. I would suggest doing a background control for each of the experimental sam...

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Thank you for contacting us.

I apologize for the wait, but I have not yet heard back from the lab. I have sent another email to follow up with them.

Thank you for your patience and understanding.

Thank you for contacting us.

I have checked with the lab and their advice is as follows:

For most efficient results, we always recommend that the samples be made in the assay buffer provided with the kit. In case of that not being...

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Thank you for contacting us.

I can confirm that the assay buffers in both the kits are different. These cannot be used interchangeably. If you are running out of buffers then let us know we can provide them separately.

I hope this...

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Thank you for your questions. My colleague is out of the office today but I would like to forward the response that we received from the lab:

Unfortunately, these three kits have different Assay buffers and converting enzyme mixes, so they ca...

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Thank you for your inquiry. This kit has not been tested with bacterial cells by the lab, however theoretically as long as the cells are prepared as indicated in the attached data sheet, it should work with such samples.  I hope this information ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"