ab54402 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Dot: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Detects a band of approximately 40 kDa for the recombinant fragment (predicted molecular weight for the full length protein: 124 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.