概述

  • 产品名称
  • 描述
    兔多克隆抗体to ADK
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human, Chinese hamster
    预测可用于: Mouse, Rat, Zebrafish
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human ADK.

  • 阳性对照
    • This antibody gave a positive signal in the following lysates: RAW 264.7 Whole Cell Lysate; Human Placenta Tissue Lysate; Human Liver Tissue Lysate; HepG2 Whole Cell Lysate.

性能

应用

Our Abpromise guarantee covers the use of ab88903 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).

靶标

图片

  • All lanes : Anti-ADK antibody (ab88903) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate at 40 µg
    Lane 2 : ADK knockout HAP1 whole cell lysate at 40 µg
    Lane 3 : HepG2 whole cell lysate at 20 µg

    Predicted band size: 41 kDa



    Lanes 1 - 3: Merged signal (red and green). Green - ab88903 observed at 41 kDa. Red - loading control, ab130007, observed at 130 kDa.

    ab88903 was shown to recognize ADK in wild-type HAP1 cells as signal was lost at the expected MW in ADK knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and ADK knockout samples were subjected to SDS-PAGE. Ab88903 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • All lanes : Anti-ADK antibody (ab88903) at 1 µg/ml

    Lane 1 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate
    Lane 2 : Human placenta tissue lysate - total protein (ab29745)
    Lane 3 : Human liver tissue lysate - total protein (ab29889)
    Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 41 kDa
    Observed band size: 39,41 kDa (why is the actual band size different from the predicted?)


    Exposure time: 8 minutes


    Ab88903 is raised against an immunogen that is present in both isoforms of ADK. We hypothesise that the bands observed correspond to the long and short isoforms, which are 41- and 39-kDa respectively.

实验方案

文献

This product has been referenced in:
  • Martínez-Vega R  et al. Folic acid deficiency induces premature hearing loss through mechanisms involving cochlear oxidative stress and impairment of homocysteine metabolism. FASEB J 29:418-32 (2015). Read more (PubMed: 25384423) »

See 1 Publication for this product

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