重组
RabMAb

Anti-Adipose Triglyceride Lipase抗体[EPR3444(2)] (ab109251)

概述

  • 产品名称
    Anti-Adipose Triglyceride Lipase抗体[EPR3444(2)]
    参阅全部 Adipose Triglyceride Lipase 一抗
  • 描述
    兔单克隆抗体[EPR3444(2)] to Adipose Triglyceride Lipase
  • 经测试应用
    适用于: WBmore details
    不适用于: Flow Cyt,ICC/IF or IHC-P
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Adipose Triglyceride Lipase aa 350-450. The exact sequence is proprietary.

  • 阳性对照
    • WB: 3T3-L1, A431 and A673 cell lysates, human heart, skeletal muscle and adipose tissue lysates. Mouse and rat heart tissue lysates.
  • 常规说明

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab109251 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 54 kDa (predicted molecular weight: 55 kDa).
  • 应用说明
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • 靶标

    • 功能
      Catalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets. Also has acylglycerol transacylase activity. May act coordinately with LIPE/HLS within the lipolytic cascade. Regulates adiposome size and may be involved in the degradation of adiposomes. May play an important role in energy homeostasis. May play a role in the response of the organism to starvation, enhancing hydrolysis of triglycerides and providing free fatty acids to other tissues to be oxidized in situations of energy depletion.
    • 组织特异性
      Highest expression in adipose tissue. Also detected in heart, skeletal muscle, and portions of the gastrointestinal tract. Detected in normal retina and retinoblastoma cells. Detected in retinal pigment epithelium and, at lower intensity, in the inner segments of photoreceptors and in the ganglion cell layer of the neural retina (at protein level).
    • 通路
      Glycerolipid metabolism; triacylglycerol degradation.
    • 疾病相关
      Note=Genetic variations in PNPLA2 may be associated with risk of diabetes mellitus type 2.
      Defects in PNPLA2 are the cause of neutral lipid storage disease with myopathy (NLSDM) [MIM:610717]; also known as neutral lipid storage disease without ichthyosis. NSLDM is a neutral lipid storage disorder (NLSD) with myopathy but without ichthyosis. NLSDs are characterized by the presence of triglyceride-containing cytoplasmic droplets in leukocytes and in other tissues, including bone marrow, skin, and muscle. Individuals with NLSDM did not show obesity, in spite of a defect in triglyceride degradation in fibroblasts and in marked triglyceride storage in liver, muscles, and other visceral cells.
    • 序列相似性
      Contains 1 patatin domain.
    • 发展阶段
      Induced during differentiation of primary preadipocytes to adipocytes. Expression increased from fetal to adult in retinal pigment epithelium.
    • 细胞定位
      Lipid droplet. Cell membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 1110001C14Rik antibody
      • Adipose triglyceride lipase antibody
      • ATGL antibody
      • ATGL DESNUTRIN antibody
      • Calcium independent phospholipase A2 antibody
      • Calcium-independent phospholipase A2 antibody
      • Desnutrin antibody
      • EC 3.1.1.3 antibody
      • FP17548 antibody
      • IPLA2 zeta antibody
      • IPLA2-zeta antibody
      • Mutant patatin like phospholipase domain containing 2 antibody
      • Patatin like phospholipase domain containing 2 antibody
      • PATATIN LIKE PHOSPHOLIPASE DOMAIN CONTAINING PROTEIN 2 antibody
      • Patatin-like phospholipase domain-containing protein 2 antibody
      • PEDF R antibody
      • PHOSPHOLIPASE A2 CALCIUM INDEPENDENT ZETA antibody
      • Pigment epithelium derived factor antibody
      • Pigment epithelium-derived factor antibody
      • plpl antibody
      • plpl2 antibody
      • PLPL2_HUMAN antibody
      • Pnpla2 antibody
      • Transport secretion protein 2 antibody
      • Transport secretion protein 2.2 antibody
      • Transport-secretion protein 2 antibody
      • Triglyceride hydrolase antibody
      • TTS 2.2 antibody
      • TTS2 antibody
      • TTS2.2 antibody
      • ZETA antibody
      see all

    Anti-Adipose Triglyceride Lipase antibody [EPR3444(2)] 图像

    • All lanes : Anti-Adipose Triglyceride Lipase antibody [EPR3444(2)] (ab109251) at 1/5000 dilution (purified)

      Lane 1 : Mouse heart tissue lysate
      Lane 2 : Rat heart tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      HRP-conjugated goat anti-rabbit IgG (specific to the non-reduced form of IgG) at 1/1000 dilution

      Predicted band size : 55 kDa
      Observed band size : 54 kDa (why is the actual band size different from the predicted?)

      Blocking and dilution buffer: 5% NFDM /TBST.

    • All lanes : Anti-Adipose Triglyceride Lipase antibody [EPR3444(2)] (ab109251) at 1/1000 dilution (purified)

      Lane 1 : Human heart tissue lysate
      Lane 2 : A431 whole cell lysate
      Lane 3 : A673 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      HRP-conjugated goat anti-rabbit IgG (specific to the non-reduced form of IgG) at 1/1000 dilution

      Predicted band size : 55 kDa
      Observed band size : 54 kDa (why is the actual band size different from the predicted?)

      Blocking and dilution buffer: 5% NFDM /TBST.

    • All lanes : Anti-Adipose Triglyceride Lipase antibody [EPR3444(2)] (ab109251) at 1/1000 dilution (unpurified)

      Lane 1 : 3T3-L1 lysate
      Lane 2 : A431 lysate
      Lane 3 : A673 lysate
      Lane 4 : Human heart tissue lysate
      Lane 5 : Human skeletal muscle tissue lysate
      Lane 6 : Human adipose tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size : 55 kDa
      Observed band size : 54 kDa (why is the actual band size different from the predicted?)

    实验方案

    Anti-Adipose Triglyceride Lipase antibody [EPR3444(2)] (ab109251)参考文献

    This product has been referenced in:
    • Hirschmugl B  et al. Maternal obesity modulates intracellular lipid turnover in the human term placenta. Int J Obes (Lond) 41:317-323 (2017). WB ; Human . Read more (PubMed: 27780978) »
    • Gao S  et al. Effects and Molecular Mechanism of GST-Irisin on Lipolysis and Autocrine Function in 3T3-L1 Adipocytes. PLoS One 11:e0147480 (2016). WB . Read more (PubMed: 26799325) »

    See all 5 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Kidney)
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Loading amount
    50 µg
    Specification
    Kidney
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Apr 05 2017

    Application
    Western blot
    Sample
    Pig Tissue lysate - whole (Liver)
    Gel Running Conditions
    Reduced Denaturing (12 %)
    Loading amount
    60 µg
    Specification
    Liver
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 7.5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    提交于 Feb 27 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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