Anti-Adiponectin Receptor 2抗体(ab77612)


  • 产品名称Anti-Adiponectin Receptor 2抗体
    参阅全部 Adiponectin Receptor 2 一抗
  • 描述
    山羊多克隆抗体to Adiponectin Receptor 2
  • 特异性Recognizes Human, Rat, Mouse and Macaca ADPR2 Receptor. May cross react with ADPR1 Receptor.
  • 经测试应用适用于: WB, IHC-P, ICC, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Macaque Monkey
    预测可用于: Chicken, Cow, Pig, Xenopus laevis
  • 免疫原

    Synthetic peptide:


    , corresponding to C terminal amino acids 362-380 of Human Adiponectin Receptor 2

  • 阳性对照
    • Human liver and human muscle tissue
  • 常规说明

    This antibody may be used in developing sandwich ELISA assays in combination with antibodies specific to N terminus of ADPR2 Receptor




Our Abpromise guarantee covers the use of ab77612 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 44 kDa.
IHC-P 1/250.

Buffer used was: 10 mM KHPO4, 140 mM NaCl, pH 7.2.

ICC 1/400.

Acetone fixed cells.

Flow Cyt Use at an assay dependent concentration. ab37373-Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.


  • 功能Receptor for globular and full-length adiponectin (APM1), an essential hormone secreted by adipocytes that acts as an antidiabetic. Probably involved in metabolic pathways that regulate lipid metabolism such as fatty acid oxidation. Mediates increased AMPK, PPARA ligand activity, fatty acid oxidation and glucose uptake by adiponectin. Has some intermediate-affinity receptor activity for both globular and full-length adiponectin.
  • 组织特异性Highly expressed in skeletal muscle, liver and placenta. Weakly expressed in brain, heart, colon, spleen, kidney, thymus, small intestine, peripheral blood leukocytes and lung.
  • 序列相似性Belongs to the ADIPOR family.
  • 结构域The N-terminus is known to be cytoplasmic while the C-terminus is known to be extracellular.
  • 细胞定位Membrane. Localized to the cell membrane and intracellular organelles.
  • Information by UniProt
  • 数据库链接
  • 别名
    • PAQR2 antibody
    • ACDCR2 antibody
    • Adiponectin receptor protein 2 antibody
    • Adipor2 antibody
    • ADR2_HUMAN antibody
    • Progestin and adipoQ receptor family member II antibody
    see all

Anti-Adiponectin Receptor 2 antibody 图像

  • Paraffin section of human liver tissue stained with antibody to human ADPR2 receptor C-terminus ab77612 at 1/250 dilution.
  • Paraffin section of human muscle tissue stained with antibody to human ADPR2 receptor C-terminus ab77612 at 1/250 dilution.
  • Anti-Adiponectin Receptor 2 antibody (ab77612) at 1 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

    Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 44 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)

    Exposure time : 20 minutes

Anti-Adiponectin Receptor 2 antibody (ab77612)参考文献

This product has been referenced in:
  • Fang F  et al. Adiponectin attenuates angiotensin II-induced oxidative stress in renal tubular cells through AMPK and cAMP-Epac signal transduction pathways. Am J Physiol Renal Physiol 304:F1366-74 (2013). Read more (PubMed: 23535586) »
  • Gulli RA  et al. Exercise restores insulin, but not adiponectin, response in skeletal muscle of high-fat fed rodents. Am J Physiol Regul Integr Comp Physiol 303:R1062-70 (2012). Rat . Read more (PubMed: 23054173) »

See all 2 Publications for this product

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As you have seen on the datasheets for the anti-Adiponectin Receptor 2 antibody (ab77612) and anti-Adiponectin Receptor 1 antibody [EPR6626] (ab126611) have both been used in flow ...

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Application Western blot
Sample Pig Cell lysate - whole cell (porcine skeletal muscle)
Loading amount 50 µg
Specification porcine skeletal muscle
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step RotiBlock (Carl Roth GmbH & Co. KG, Karlsruhe, Germany) in TBS (Tris buffered saline, 200 mM Tris, 1.37 M NaCl, pH 7.4) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C

Abcam user community

Verified customer

提交于 Aug 22 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Liver)
Specification Liver
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step Normal goat serum as blocking agent for 15 minute(s) · Concentration: 10% · Temperature: 25°C

Abcam user community

Verified customer

提交于 Apr 08 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (mesothelioma cell line (ATCC: MSTO-211H))
Specification mesothelioma cell line (ATCC: MSTO-211H)
Preparation Cell harvesting/tissue preparation method: 10% FCS / RPMI 1640
Sample buffer: 2% FCS / PBS
Fixation non-fixation
Permeabilization No
Gating Strategy R1 and R2

Abcam user community

Verified customer

提交于 Feb 22 2010