Adipogenesis Detection Assay试剂盒(Colorimetric/Fluorometric) (ab102513)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 40 min
- Sample type: Adherent cells, Suspension cells, Tissue
- Sensitivity: 0.2 nM
概述
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产品名称
Adipogenesis Detection Assay试剂盒(Colorimetric/Fluorometric)
参阅全部 Adipogenesis 试剂盒 -
检测方法
Colorimetric/Fluorometric -
样品类型
Tissue, Adherent cells, Suspension cells -
检测类型
Quantitative -
灵敏度
> 0.2 nM -
范围
0.2 nM - 10 nM -
检测时间
0h 40m -
产品概述
Adipogenesis Detection Assay Kit (Colorimetric/Fluorometric) (ab102513) quantifies triglyceride accumulation in cells and tissues. In the assay, triglycerides are efficiently solubilized then hydrolyzed to glycerol which is subsequently oxidized to convert the probe to generate color (ODmax = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The kit can detect 0.2 - 10 nmol of triglyceride in <1,000 differentiated 3T3-L1 cells. The high detection sensitivity and the convenient microplate assay format make the kit a convenient tool for studying the effect of adipogenesis inducers or to screen inhibitor compounds.
Visit our FAQs page for tips and troubleshooting.
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说明
This product is manufactured by BioVision, an Abcam company and was previously called K610 Adipogenesis Colorimetric/Fluorometric Assay Kit. K610-100 is the same size as the 100 test size of ab102513.
Adipogenesis is the process of differentiation of different cell types into adipocytes, the primary fat storage cell type. The accumulation of adipocytes is the basis for obesity, a significant risk factor in many diseases, including diabetes, atherosclerosis, cancer and cardiovascular disease, etc. Adipocytes accumulate triglycerides, in the form of lipid droplets which can be measured.
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平台
Microplate reader
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 100 tests 2000 tests Adipogenesis Lipid Extraction Solution 1 x 10ml 20 x 10ml Assay Buffer V 1 x 25ml 20 x 25ml Enzyme Mix VI 1 vial 20 vials Lipase I 1 vial 20 vials OxiRed Probe 1 x 200µl 20 x 200µl Triglyceride Standard 1 x 0.3ml 20 x 0.3ml -
研究领域
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相关性
Adipogenesis is the process of differentiation of different cell types into adipocytes, the primary fat storage cell type. The accumulation of adipocytes is the basis for obesity, a significant risk factor in many diseases, including diabetes, atherosclerosis, cancer and cardiovascular disease, etc. Adipocytes accumulate triglycerides, in the form of lipid droplets which can be measured.
相关产品
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Related Products
图片
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Example of a standard curve obtained with ab102513.
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Example of a standard curve obtained with ab102513.
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Glycerol measured fluorometrically in mouse tissue lysates showing quantity (nmol) per mg protein of tested sample
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Glycerol measured colourimetrically in mouse tissue lysates showing quantity (nmol) per mg protein of tested sample.
Ms brain value retrieved from 1:9 dilution of original 12.4mg/mL sample.
Ms muscle value retrieved from 1:9 dilution of original 7.5mg/mL sample.
Ms heart value retrieved from 1:27 dilution of original 16.4mg/mL sample.
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Glycerol measured colourimetrically in cell lysates showing quantity (nmol) per 1 mln of tested cells
数据表及文件
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SDS download
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Datasheet download
文献 (15)
ab102513 被引用在 15 文献中.
- Goyal P et al. Physicochemical and functional characterization of MYL-1501D, a proposed biosimilar to insulin glargine. PLoS One 16:e0253168 (2021). PubMed: 34133466
- Lee JS et al. Social isolation-related depression accelerates ethanol intake via microglia-derived neuroinflammation. Sci Adv 7:eabj3400 (2021). PubMed: 34739315
- Jung YJ et al. Cell reprogramming using extracellular vesicles from differentiating stem cells into white/beige adipocytes. Sci Adv 6:eaay6721 (2020). PubMed: 32232152
- Zhou Y et al. Maternal diet intervention before pregnancy primes offspring lipid metabolism in liver. Lab Invest 100:553-569 (2020). PubMed: 31748681
- Stelling E et al. Increased prostaglandin-D2 in male STAT3-deficient hearts shifts cardiac progenitor cells from endothelial to white adipocyte differentiation. PLoS Biol 18:e3000739 (2020). PubMed: 33370269