Anti-ADH5抗体(ab91385)

概述

  • 产品名称Anti-ADH5抗体
    参阅全部 ADH5 一抗
  • 描述
    兔多克隆抗体to ADH5
  • 经测试应用适用于: ICC/IF, WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide selected from the center region of Human ADH5, conjugated to KLH (NP_000662.3).

  • 阳性对照
    • K562 cell lysate IF/ICC: HeLa cell line

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab91385 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF
WB
ELISA
  • 应用说明ELISA: 1/1000.
    WB: 1/100 - 1/500. Predicted molecular weight: 40 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Class-III ADH is remarkably ineffective in oxidizing ethanol, but it readily catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione.
    • 序列相似性Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily.
    • 细胞定位Cytoplasm.
    • Information by UniProt
    • 数据库链接
    • 别名
      • ADH 3 antibody
      • ADH5 antibody
      • ADHX antibody
      • ADHX_HUMAN antibody
      • Alcohol dehydrogenase (class III) chi polypeptide antibody
      • alcohol dehydrogenase 5 (class III) chi polypeptide antibody
      • Alcohol dehydrogenase 5 antibody
      • Alcohol dehydrogenase class 3 antibody
      • Alcohol dehydrogenase class chi chain antibody
      • Alcohol dehydrogenase class III antibody
      • Alcohol dehydrogenase class-3 antibody
      • Alcohol dehydrogenase class-III antibody
      • class III alcohol dehydrogenase 5 chi subunit antibody
      • FALDH antibody
      • FDH antibody
      • formaldehyde dehydrogenase antibody
      • Glutathione dependent formaldehyde dehydrogenase antibody
      • Glutathione-dependent formaldehyde dehydrogenase antibody
      • GSH-FDH antibody
      • GSNOR antibody
      • hydroxymethyllutathione dehydrogenase antibody
      • S-(hydroxymethyl)glutathione dehydrogenase antibody
      see all

    Anti-ADH5 antibody 图像

    • ICC/IF image of ab91385 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab91385, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

    • Anti-ADH5 antibody (ab91385) at 1/100 dilution + K562 cell lysate at 35 µg

      Predicted band size : 40 kDa

    Anti-ADH5 antibody (ab91385)参考文献

    ab91385 has not yet been referenced specifically in any publications.

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