概述

  • 产品名称Anti-ADFP抗体
    参阅全部 ADFP 一抗
  • 描述
    兔多克隆抗体to ADFP
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Pig, Monkey
  • 免疫原

    A synthetic peptide made to an internal portion of the human ADFP protein, within residues 150-250.

  • 阳性对照
    • Human liver lysate

应用

Our Abpromise guarantee covers the use of ab52355 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. PubMed: 19920176
WB Use a concentration of 2 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).

靶标

Anti-ADFP antibody 图像

  • Anti-ADFP antibody (ab52355) at 2 µg/ml + Human liver lysate

    Predicted band size : 48 kDa
    Observed band size : 48 kDa
  • ab52355 staining ADFP in HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% saponin and blocked with 2% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/50: in PBS; 1% BSA) for 1 hour at 25°C. An Alexa Fluor®568-conjugated Goat polyclonal to rabbit IgG, dilution 1/100, was used as secondary antibody.

    See Abreview

Anti-ADFP antibody (ab52355)参考文献

This product has been referenced in:
  • Akil A  et al. Septin 9 induces lipid droplets growth by a phosphatidylinositol-5-phosphate and microtubule-dependent mechanism hijacked by HCV. Nat Commun 7:12203 (2016). WB, ICC/IF ; Human . Read more (PubMed: 27417143) »
  • Eggert D  et al. Visualization and analysis of hepatitis C virus structural proteins at lipid droplets by super-resolution microscopy. PLoS One 9:e102511 (2014). ICC/IF . Read more (PubMed: 25019511) »

See all 7 Publications for this product

Product Wall

Application Western blot
Loading amount 1 µg
Gel Running Conditions Reduced Denaturing (4-12% gradient)
Sample Human Cell lysate - whole cell (Huh7 liver)
Specification Huh7 liver
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Nov 14 2014

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.
The details you have kindly provided will enable us to investigate this case ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0,1% saponin
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Dr. Francesca Lolicato

Verified customer

提交于 Jan 29 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"