Anti-Adenylate cyclase 1抗体(ab69597)


  • 产品名称Anti-Adenylate cyclase 1抗体
    参阅全部 Adenylate cyclase 1 一抗
  • 描述
    兔多克隆抗体to Adenylate cyclase 1
  • 特异性Detects endogenous levels of total Adenylate cyclase 1 protein.
  • 经测试应用适用于: IHC-P, WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide (Human) derived from an internal region of human Adenylate cyclase 1.

  • 阳性对照
    • Extracts from COLO205 cells



Our Abpromise guarantee covers the use of ab69597 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Detects a band of approximately 130 kDa (predicted molecular weight: 124 kDa).
ELISA 1/40000.


  • 功能This is a calmodulin-sensitive adenylyl cyclase. May be involved in regulatory processes in the central nervous system. It may play a role in memory acquisition and learning.
  • 组织特异性Brain, retina and adrenal medulla.
  • 序列相似性Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.
    Contains 2 guanylate cyclase domains.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Adcy1 antibody
    • ADCY1_HUMAN antibody
    • adenyl cyclase antibody
    • Adenylate cyclase 1 antibody
    • Adenylate cyclase type 1 antibody
    • Adenylate cyclase type I antibody
    • Adenylyl cyclase 1 antibody
    • ATP pyrophosphate lyase antibody
    • ATP pyrophosphate-lyase 1 antibody
    • Brain adenylate cyclase 1 antibody
    • Ca(2+)/calmodulin-activated adenylyl cyclase antibody
    see all

Anti-Adenylate cyclase 1 antibody 图像

  • All lanes : Anti-Adenylate cyclase 1 antibody (ab69597) at 1/500 dilution

    Lane 1 : Extracts from COLO205
    Lane 2 : Extracts from COLO205 with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.

    Predicted band size : 124 kDa
    Observed band size : 130 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 105 kDa,150 kDa,85 kDa. We are unsure as to the identity of these extra bands.
  • IHC image of ab69597 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab69597, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Adenylate cyclase 1 antibody (ab69597)参考文献

This product has been referenced in:
  • Tekmen M & Gleason E Multiple Ca2+-dependent mechanisms regulate L-type Ca2+ current in retinal amacrine cells. J Neurophysiol 104:1849-66 (2010). WB ; Chicken . Read more (PubMed: 20685929) »

See 1 Publication for this product

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Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

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TThank you for contacting us.

We do offer a number of products against the 10 Adenylate cyclase types however we do not currently have any products which will recognize all forms of this protein.

I have linked both a Unipro...

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