Anti-Adenovirus Type 5 E1A抗体[M58] (ab76552)

概述

  • 产品名称Anti-Adenovirus Type 5 E1A抗体[M58]
    参阅全部 Adenovirus Type 5 E1A 一抗
  • 描述
    小鼠单克隆抗体[M58] to Adenovirus Type 5 E1A
  • 经测试应用适用于: WB, IP, IHC-P, ICC/IFmore details
  • 种属反应性
    Adenovirus
  • 免疫原

    Adenovirus.

  • 阳性对照
    • 293 cells or adenovirus-infected cells and tissues.

性能

应用

Our Abpromise guarantee covers the use of ab76552 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
IHC-P
ICC/IF
  • 应用说明ICC/IF: Use at a concentration of 0.2 - 0.4 µg/ml.
    IHC-P: Use at a concentration of 2 - 4 µg/ml. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min
    followed by cooling at room temperature for 20 min.
    IP: Use at 2µg/mg of lysate.
    WB: Use at a concentration of 0.5 - 1.0 µg/ml. Predicted molecular weight: 32 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性The early region (E1A) of the adenovirus genome plays a central role in cellular transformation and regulation of gene expression. The E1A region encodes a series of related proteins (35-46kDa) with multifuctional capabilities and form a specific complex with the retinoblastoma tumor suppressor gene product (Rb protein). The E1A and E1B regions together comprise the transforming region of adenovirus. While expression of E1A alone is sufficient to immortalize primary cells, complete transformation requires the additional expression of the E1B region. Several conserved regions of E1A are similar to portions of other viral oncoproteins like the HPV-16 and HPV-18 E7 and SV40 large T antigen.
    • 别名
      • Hadv 5 E1A antibody
      • HAdv5 E1A antibody

    Anti-Adenovirus Type 5 E1A antibody [M58] 图像

    • Anti-Adenovirus Type 5 E1A antibody [M58] (ab76552) at 0.5 µg/ml + Human cells

      Predicted band size : 32 kDa

    Anti-Adenovirus Type 5 E1A antibody [M58] (ab76552)参考文献

    ab76552 has not yet been referenced specifically in any publications.

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