Anti-Adenovirus Type 5 E1A抗体[M58] (ab31686)

概述

  • 产品名称
    Anti-Adenovirus Type 5 E1A抗体[M58]
    参阅全部 Adenovirus Type 5 E1A 一抗
  • 描述
    小鼠单克隆抗体[M58] to Adenovirus Type 5 E1A
  • 特异性
    ab31686 recognises Adenovirus, Type 5. The clone number has been updated from (0.N.8) to (M58) both clone numbers name the same antibody clone.
  • 经测试应用
    适用于: IHC-P, IHC-FoFr, ICC/IF, IP, WB, IHC-Frmore details
  • 种属反应性
    Adenovirus.
  • 免疫原

    Tissue/ cell preparation Adenovirus.

  • 阳性对照
    • 293 cells or adenovirus infected cells and tissues.

性能

应用

Our Abpromise guarantee covers the use of ab31686 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Incubate for 2 hours at room temperature.
IHC-FoFr Use a concentration of 2 - 4 µg/ml. Perform antigen retrieval before commencing the IHC protocol. Incubate for 2 hours at room temperature.
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 35-46 kDa. Incubate for 2 hours at room temperature.
IHC-Fr Use at an assay dependent concentration.

靶标

  • 相关性
    The early region (E1A) of the adenovirus genome plays a central role in cellular transformation and regulation of gene expression. The E1A region encodes a series of related proteins (35-46kDa) with multifuctional capabilities and form a specific complex with the retinoblastoma tumor suppressor gene product (Rb protein). The E1A and E1B regions together comprise the transforming region of adenovirus. While expression of E1A alone is sufficient to immortalize primary cells, complete transformation requires the additional expression of the E1B region. Several conserved regions of E1A are similar to portions of other viral oncoproteins like the HPV-16 and HPV-18 E7 and SV40 large T antigen.
  • 别名
    • Hadv 5 E1A antibody
    • HAdv5 E1A antibody

Anti-Adenovirus Type 5 E1A antibody [M58] (ab31686)参考文献

This product has been referenced in:
  • Saha B & Parks RJ Human adenovirus type 5 vectors deleted of early region 1 (E1) undergo limited expression of early replicative E2 proteins and DNA replication in non-permissive cells. PLoS One 12:e0181012 (2017). WB ; Human . Read more (PubMed: 28700677) »
  • Turowski G  et al. Viral infection in placenta relevant cells - a morphological and immunohistochemical cell culture study. APMIS N/A:N/A (2014). Read more (PubMed: 25244625) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK 293)
Permeabilization
Yes - 0.1% Triton X100
Specification
HEK 293
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Apr 03 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Syrian Hamster Tissue sections (Brain. Infected NSC-GFP with adenovirus were injec)
Specification
Brain. Infected NSC-GFP with adenovirus were injec
Fixative
Methanol
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Bart Thaci

Verified customer

提交于 May 12 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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