Anti-Adenovirus Type 5抗体(ab6982)

概述

  • 产品名称
    Anti-Adenovirus Type 5抗体
  • 描述
    兔多克隆抗体to Adenovirus Type 5
  • 经测试应用
    适用于: Neutralising, IHC-P, IHC-Fr, ELISA, WB, ICC/IFmore details
  • 免疫原

    Purified Adenovirus type 5

性能

应用

Our Abpromise guarantee covers the use of ab6982 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Neutralising Use at an assay dependent concentration. This antibody will neutralize Ad5 infection in a bioassay (1/25,000 dilution for 50 % inactivation of Ad5at 1000 particles/ml).
IHC-P Use at an assay dependent concentration. PubMed: 20442708
IHC-Fr Use at an assay dependent concentration. PubMed: 20442708
ELISA Use at an assay dependent concentration.

Recognizes Ad5 immobilized on microtiter plates.

WB Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

靶标

  • 相关性
    Adenoviruses are DNA viruses that cause upper respiratory tract infections, conjunctivitis, and other infections in humans. Adenoviruses were discovered in 1953. About 47 different types have been identified since then, and about half of them are believed to cause human diseases. Infants and children are most commonly affected by adenoviruses. Adenovirus infections can occur throughout the year, but seem to be most common from fall to spring. Adenoviruses are responsible for 3-5% of acute respiratory infections in children and 2% of respiratory illnesses in adults.
  • 别名
    • Adenovirus 5 antibody
    • AdV 5 antibody

Anti-Adenovirus Type 5 antibody 图像

  • ab6982 staining Adenovirus type 5 in human HeLa cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.1% Triton and then blocked using 0.1% BSA for 16 hours at 4°C. Samples were then incubated with primary antibody at 1/800 for 1 hour at 37°C. The secondary antibody used was a donkey anti-rabbit IgG conjugated to Alexa Fluor® 546 (red) used at a 1/1000 dilution.

    See Abreview

  • Top blot (A): ab6982 at a 1/4000 dilution staining Adenovirus type 5 capsid proteins by Western blot (ECL). Secondary = goat anti-rabbit (HRP conjuagte).

    Bottom blot (B): As above, except ab6982 omitted.

    Top blot (A): ab6982 at a 1/4000 dilution staining Adenovirus type 5 capsid proteins by Western blot (ECL). Secondary = goat anti-rabbit (HRP conjuagte). Bottom blot (B): As above, except ab6982 omitted.
  • Additional information regarding Adenovirus type 5 capsid proteins. 
  • All lanes : Anti-Adenovirus Type 5 antibody (ab6982) at 1/10000 dilution

    Lane 1 : human hepatocytes whole cell lysate
    Lane 2 : human hepatocytes whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP conjugated goat anti-rabbit secondary polyclonal antibody at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 130 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 seconds

    Image courtesy of an anonymous Abreview

    Blocked with 5% milk

    See Abreview

  • All lanes : Anti-Adenovirus Type 5 antibody (ab6982) at 1/10000 dilution

    Lane 1 : Whole cell lysate prepared from U2OS cells.
    Lane 2 : Whole cell lysate prepared from U2OS cells infected with Adenovirus 5.

    Lysates/proteins at 35 µg per lane.

    Secondary
    Goat anti-rabbit conjugated to IRDye 700DX

    Image courtesy of an anonymous Abreview.

    Blocking step was performed using 5% milk for 1 hour at 23°C.

    See Abreview

Anti-Adenovirus Type 5 antibody (ab6982)参考文献

This product has been referenced in:
  • Scherer J & Vallee RB Conformational changes in the adenovirus hexon subunit responsible for regulating cytoplasmic dynein recruitment. J Virol 89:1013-23 (2015). Read more (PubMed: 25355895) »
  • Tsou YL  et al. Recombinant adeno-vaccine expressing enterovirus 71-like particles against hand, foot, and mouth disease. PLoS Negl Trop Dis 9:e0003692 (2015). Read more (PubMed: 25855976) »

See all 27 Publications for this product

Product Wall

Application
Western blot
Sample
Human Cell lysate - whole cell (human hepatocytes)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Treatment
infected with Adv-iNOS
Specification
human hepatocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Mar 31 2017


ab6982 is a plyclonal antibody and as such shows recognition to every protein in the Ad5 capsid, including the knob.

Thank you very much for your reply.

If the specific signal is fairly strong, you can further dilute the antibody to 1:200-1:1000 which should help reduce non-specific binding. You could also try blocking the tissue overnight at 4C, and contin...

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Thank you very much for contacting us with your question, and for your patience while I have been in touch with the lab to request more information about this antibody.

The antibody was validated in IHC according to the following publication,...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (AdV-infected submandibular salivary gland)
Specification
AdV-infected submandibular salivary gland
Fixative
Paraformaldehyde
Permeabilization
Yes - tritonX 0.2%
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 10% · Temperature: 15°C
Username

Abcam user community

Verified customer

提交于 Oct 02 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Hek293)
Loading amount
25 µg
Specification
Hek293
Treatment
AdV-infected
Gel Running Conditions
Non-reduced Denaturing (4-12% Tris-Bis)
Blocking step
Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Oct 02 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Liver)
Specification
Liver
Fixative
Acetone
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 20% · Temperature: 15°C
Username

Abcam user community

Verified customer

提交于 Aug 24 2012

Thank you for calling Abcam earlier today.

I am sorry that you are not getting any signal using the current lot of ab6982. As we discussed, I am sending you a new vial of ab6982 to use. Your new order number is */.

If you have any dif...

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Thank you for contacting us.

Yes, I can confirm that "Type 5" is a shortened version of the more scientifically precise term serotype 5. They both mean exactly the same.All Adenovirus classification is based upon serotyping. This antibody ab...

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Thank you for contacting us.

We do not have this antibody inbiotinylated form, but we do have conjugation kits for biotinylation:

http://www.abcam.com/easylink

Biotin type A (e.g. ab102865 (3x10 ug) and other sizes)
...

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1-10 of 31 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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