Anti-Adenovirus hexon蛋白抗体[8C4] (ab8249)

Abreviews (3)Q&A (8)Specific References (3)

概述

  • 产品名称
    Anti-Adenovirus hexon蛋白抗体[8C4]
    参阅全部 Adenovirus hexon protein 一抗
  • 描述
    小鼠单克隆抗体[8C4] to Adenovirus hexon蛋白
  • 宿主
    Mouse
  • 特异性
    Not tested with adenovirus type 5.
  • 经测试应用
    适用于: Sandwich ELISA, ELISA, Immunodiffusion, Flow Cyt, ICCmore details
  • 免疫原

    Hexon antigen of human adenovirus

  • 阳性对照
    • adenovirus-infected cells

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    PBS with 0.1% sodium azide, pH 7.4
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 纯化说明
    Purified by chromatography on protein A Sepharose. Purity is tested by electrophoresis.
  • 克隆
    单克隆
  • 克隆编号
    8C4
  • 同种型
    IgG2a
  • 轻链类型
    unknown
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab8249 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [1E11] to Adenovirus hexon protein (ab8251). Can be paired with Adenovirus hexon protein antibody [1E11] (ab8251).
ELISA Use at an assay dependent concentration.
Immunodiffusion Use at an assay dependent concentration.
Flow Cyt Use 1µg for 105 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 
ICC Use at an assay dependent concentration.

靶标

  • 相关性
    Hexon protein is a major coat protein of adenoviruses. Adenoviruses capsids have three principal protein components: the hexon, the penton, and the fiber. Hexon consists of three subunits together forming two major components of different morphological symmetry. A triangular top with three towers of density is superimposed on a more bulky pseudo hexagonal base. The symmetry of the top is in accord with the trimeric nature of hexon, but that of the base derives from the molecular function, which is to provide a densely packed impenetrable protective outer layer for the virion.
  • 细胞定位
    Major coat protein of adenoviruses.
  • 别名
    • Hexon protein antibody
    • Late protein 2 antibody
    • PII antibody

图片

  • Immunocytochemistry - Anti-Adenovirus hexon protein antibody [8C4] (ab8249)
    Immunocytochemistry - Anti-Adenovirus hexon protein antibody [8C4] (ab8249)
    Ab8249 (1/1000) positively staining methanol fixed Hek293 cells infected with Adenovirus serotype 5.

    Secondary: Goat anti mouse HRP (1/1000), incubation was carried out for 1 hr at room temperature.

    Detection system: DAB

    Negative Control: Non transfected Hek293 cells

    This image is courtesy of an Abreview submitted on 25 October 2005. We do not have any further information relating to this image.

    See Abreview

文献

This product has been referenced in:
  • Dong W  et al. ORCA-010, a novel potency-enhanced oncolytic adenovirus, exerts strong antitumor activity in preclinical models. Hum Gene Ther 25:897-904 (2014). Read more (PubMed: 25093639) »
  • Hsieh JL  et al. Transthyretin-driven oncolytic adenovirus suppresses tumor growth in orthotopic and ascites models of hepatocellular carcinoma. Cancer Sci 100:537-45 (2009). WB . Read more (PubMed: 19134007) »

See all 3 Publications for this product

发表研究结果有使用 ab8249？请让我们知道，以便我们可以引用本数据表中的参考文章。

客户评价及客户问答

提交评价 提交问题

11 reviews or Q&A

10
Votes: Highest First

Immunocytochemistry abreview for Anti-Adenovirus hexon protein antibody [8C4]

 Excellent
Abreviews
Application
Immunocytochemistry
Sample
Human Cultured Cells (hAdenovirus 48 Infected HEK293s)
Permeabilization
No
Specification
hAdenovirus 48 Infected HEK293s
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Methanol
Read More
Username

Abcam user community

Verified customer

提交于 Feb 03 2017

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Adenovirus hexon protein antibody [8C4]

 Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Human infected cell lines)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako low pH buffer
Permeabilization
No
Specification
Human infected cell lines
Blocking step
Dako Flex peroxidase block as blocking agent for 5 minute(s) · Concentration: 100%
Fixative
Paraformaldehyde
Read More
Username

Abcam user community

Verified customer

提交于 Nov 24 2015

One of our cusomers recieved a new batch from ab8249 xx
We noticed that on the data shhet the storage instructions were changed from 4 degree to 20

Pleas advise if they ncan continue storing at 4 degree and pleas provide tham also with ...

Read More

Thank you for your inquiry.

Please find in the attachment the CoC as requested for this lot.

I am sorry, I could however not find whether and why this product has been previously recommended to be stored at 4°C.

The cus...

Read More

Dear Technical Please issue coc for ab8249 from lot GR41773-1 And ab6728 from lot GR15565-7 Please don't forget to mention expiry date Best regards

Thank you for your enquiry. Please find attached the CoCs you requested. We do not have set expiration dates for our products. Most antibodies are stable and can last for anywhere from a few months to several years if stored properly, so we stron...

Read More

Dear Technical Please advise lots available for ab8249 and the expiry dates that will be written on the CoC that we will ask for them   Best regards

Thank you for contacting us with your questions. We currently have lots *** of ab8249 available in our UK stock. While we don't assign specific expiration dates for our products, if stored at 4C as indicated on the datasheet, we would expect ab8249 to ...

Read More

Immunocytochemistry abreview for Anti-Adenovirus hexon protein antibody [8C4]

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Human Cell (human Adenovirus serotype 5 in HEK293 cells)
Specification
human Adenovirus serotype 5 in HEK293 cells
Fixative
Methanol
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 1%
Read More
abreview image
Username

Abcam user community

Verified customer

提交于 Oct 25 2005

I had a few questions about your mAbs against adenovirus hexon. The products are ab8249, ab7428, and ab8251. 1) Do these Abs recognize hexon from Ad5? 2) Are these neutralizing Abs for Ad5? 3) Do they recognize hexon in intact Ad particles? If...

Read More

Thank you for your enquiry. Unfortunately I do not have details of the degree of reactivity of ab8249 and ab8251 against the Adenovirus subclasses. However, I can tell you that ab7428 recognises all Adenovirus serotypes. All antibodies are yet to...

Read More

PRE-SALES ENQUIRY Going to use this antibody for viral titering by immunohistochemical staining. What dilution have you use for this application.

Thank you for your enquiry and your interest in our products. We would like to confirm that this antibody has been tested for ELISA, Immunodiffusion applications but NOT for immunohistochemical staining. We would suggest performing a preliminar...

Read More

I would like to know if this product (ab8249) has ever been used in a Western Blot? I am looking for the presence of the Hexon Protein at 109 kda and want to make sure this antibody will be useful in this Western experiment and yield a strong signal. T...

Read More

Thank you for your enquiry. Ab8249 has not been tested in other applications than ELISA or immunodiffusion I'm afraid. This doesn't mean the antibody doesn't work in WB, but that as we haven't tested this application we cannot garantee it will work. ...

Read More

Dear Tech support, I a customer that would like to know if the mouse monoclonal [8C4] to Adenovirus hexon protein can recognize AB5 and/or AB35 in terms of the core hexon. Also, what the epitope is. Thank you in advance,

Thank you for your enquiry. The clone 8C4 is adenovirus hexon specific. The antibody is directed against a genus-specific hexon antigen, which is present in all human serotypes. Unfortunately we have not tested recognition of AB5 and AB35.

1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册