Anti-Adenosine A2b Receptor抗体(ab40002)

概述

性能

应用

Our Abpromise guarantee covers the use of ab40002 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 37 kDa).
IHC-P Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 相关性Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
  • 细胞定位Multi-pass membrane protein.
  • 数据库链接
  • 别名
    • Adenosine receptor A2b antibody
    • adora 2 antibody
    • adora 2b antibody
    • ADORA2 antibody
    • ADORA2B antibody
    • HGNC:264 antibody
    • Netrin 1 receptor antibody
    see all

Anti-Adenosine A2b Receptor antibody 图像

  • ab40002 (2µg/ml) staining of paraffin embedded Human Kidney shows cytoplasm staining in cells of renal tubules  following steamed antigen retrieval with citrate buffer pH 6 and HRP-staining.

  • Anti-Adenosine A2b Receptor antibody (ab40002) at 0.5 µg/ml + 100ug of rat kidney cortex lysate.

    Secondary
    Bovine-anti goat IgG conjugated to HRP at 1/7500 dilution

    Performed under reducing conditions.

    Predicted band size : 37 kDa

    This image is courtesy of an Abreview submitted by Dr Prit Gill

    See Abreview

  • ab40002 at 3ug/ml staining Adenosine A2b receptor in human kidney tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in microwave with Tris/EDTA buffer (pH 9.0). The HRP staining procedure was used for detection.

Anti-Adenosine A2b Receptor antibody (ab40002)参考文献

This product has been referenced in:
  • Quezada C  et al. 5'-ectonucleotidase mediates multiple-drug resistance in glioblastoma multiforme cells. J Cell Physiol 228:602-8 (2013). Read more (PubMed: 22833450) »
  • Cárdenas A  et al. Adenosine A(2B) receptor-mediated VEGF induction promotes diabetic glomerulopathy. Lab Invest : (2012). Read more (PubMed: 23069939) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TRIS EDTA Buffer pH 9,0
Sample Human Tissue sections (kidney)
Specification kidney
Permeabilization Yes - Wash Buffer from Dako with Tween
Fixative Paraformaldehyde
Username

Mr. Rudolf Jung

Verified customer

提交于 Sep 06 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Rabbit Tissue lysate - whole (Rabbit: aorta, liver, heart)
Loading amount 20 µg
Specification Rabbit: aorta, liver, heart
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Mr. Kamil Witkowski

Verified customer

提交于 Apr 09 2013

Thank you for contacting us.

The immunogen sequence homology is 90% with rabbit sequence. This antibody most likely will work.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice...

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DISCOUNT CODE: ***
Expiration date: December 9, 2012

This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview forab40002 in ICC/IFand include this code in the “Addi...

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DISCOUNT CODE: ************ - For ab4002
Expiration date: **********

I am very pleased to hear you would like to accept our offer and test ab40002 in rhesus macaque. This code will give you: 1 free PRIMARY ANTIBODY before the expiration...

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I now have some additional information to share with you in regards to the antibodies I discussed in my last email against adenosine receptors.

Anti-Adenosine Receptor A2a

Unfortunately I can confirm that the other two antibodies I ...

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Application Western blot
Sample Rat Tissue lysate - whole (Kidney Cortex)
Loading amount 100 µg
Specification Kidney Cortex
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5%
Username

Dr. prit gill

Verified customer

提交于 Apr 16 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"