Anti-Adenosine A1 Receptor抗体(ab3460)

概述

  • 产品名称Anti-Adenosine A1 Receptor抗体
    参阅全部 Adenosine A1 Receptor 一抗
  • 描述
    兔多克隆抗体to Adenosine A1 Receptor
  • 特异性Detects Adenosine Receptor A1. This antibody does not detect other AR subtypes.
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat
    预测可用于: Cow, Human
  • 免疫原

    Synthetic peptide corresponding to Rat Adenosine A1 Receptor aa 309-326.
    Sequence:

    CQPKPPIDEDLPEEKAED


    (Peptide available as ab5893)

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab3460 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).Can be blocked with Adenosine A1 Receptor peptide (ab5893).
IHC-P 1/50 - 1/200.

靶标

Anti-Adenosine A1 Receptor antibody 图像

  • ab3460 labelling Adenosine A1 receptor in the cytoplasm of Rat brain tissue (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Anti-Adenosine A1 Receptor antibody (ab3460)参考文献

This product has been referenced in:
  • Datino T  et al. Mechanisms by which adenosine restores conduction in dormant canine pulmonary veins. Circulation 121:963-72 (2010). WB . Read more (PubMed: 20159830) »
  • Kempson SA  et al. Acute inhibition of the betaine transporter by ATP and adenosine in renal MDCK cells. Am J Physiol Renal Physiol 295:F108-17 (2008). WB ; Dog . Read more (PubMed: 18448594) »

See all 2 Publications for this product

Product Wall

Following up my previous message, the immunogen for ab124780, an alternative to ab3460, is also unfortunately unlikely to react with canine Adenosine A1 Receptor. Alignment of the canine receptor amino acid sequence with that of the ab124780 immunogen ...

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Thank you for contacting us. I think the issue is probably that the homology between the immunogen and the canine receptor is low: 67% identity. We do not have a dog AAR1 to test this.

In general, for multi-pass membrane receptors, we sugges...

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Thank you for your reply. If the signal is stronger on frozen section compared to formalin-fixed sections, it indicates that the epitope unmasking may not be proper. We would suggest trying to treat the samples in microwave for different times and ...

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Thank you for your enquiry and for your patience. We are very sorry to hear that you are having problem with this antibody. We have searched our database and found that this is a popular selling product and your feedback is the first we have receive...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"