Anti-Adenomatous polyposis coli protein 2抗体(ab80018)

概述

  • 产品名称
    Anti-Adenomatous polyposis coli protein 2抗体
    参阅全部 Adenomatous polyposis coli protein 2 一抗
  • 描述
    兔多克隆抗体to Adenomatous polyposis coli protein 2
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to Human Adenomatous polyposis coli protein 2 aa 2250 to the C-terminus conjugated to keyhole limpet haemocyanin.
    Database link: O95996
    (Peptide available as ab88193)

  • 阳性对照
    • This antibody gave a positive signal in human spinal cord tissue lysate.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab80018 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution. Detects a band of approximately 213 kDa (predicted molecular weight: 243 kDa).

靶标

  • 功能
    Promotes rapid degradation of CTNNB1 and may function as a tumor suppressor. May function in Wnt signaling.
  • 组织特异性
    Widely expressed (at protein level). Specifically expressed in the CNS.
  • 序列相似性
    Belongs to the adenomatous polyposis coli (APC) family.
    Contains 6 ARM repeats.
  • 发展阶段
    Expressed in fetal brain.
  • 细胞定位
    Cytoplasm, cytoskeleton. Golgi apparatus. Cytoplasm. Cytoplasm, perinuclear region. Associated with actin filaments (PubMed:11691822). Associated with microtubule network (PubMed:10644998, PubMed:11691822).
  • Information by UniProt
  • 数据库链接
  • 别名
    • Adenomatous polyposis coli protein 2 antibody
    • Adenomatous polyposis coli protein-like antibody
    • APC-like antibody
    • apc2 antibody
    • APC2_HUMAN antibody
    • APCL antibody
    see all

Anti-Adenomatous polyposis coli protein 2 antibody 图像

  • Anti-Adenomatous polyposis coli protein 2 antibody (ab80018) at 1 µg/ml + Human spinal cord tissue lysate - total protein (ab29188) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 243 kDa
    Observed band size : 213 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 secondsab80018 detects a strong band at 213 kDa in human spinal cord tissue lysate. This antibody was raised against an immunogen that is predicted to cross react with all known isoforms of adenomatous polyposis coli protein 2 (APC2). APC2 has three isoforms, isoform one has a molecular weight of 243 kDa with isoforms two and three a molecular weight of 213 and 243 respectively(SwissProt data). The band we have observed is thought to represent isoform 2 of APC2 (213 kDa).

实验方案

Anti-Adenomatous polyposis coli protein 2 antibody (ab80018)参考文献

ab80018 has not yet been referenced specifically in any publications.

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