Anti-Adenine Nucleotide Translocase 1抗体(ab102032)

概述

  • 产品名称
    Anti-Adenine Nucleotide Translocase 1抗体
    参阅全部 Adenine Nucleotide Translocase 1 一抗
  • 描述
    兔多克隆抗体to Adenine Nucleotide Translocase 1
  • 经测试应用
    适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Sheep, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to Human Adenine Nucleotide Translocase 1 aa 35-84 (N terminal).
    Sequence:

    LLQVQHASKQISAEKQYKGIIDCVVRIPKEQGFLSFWRGNLANVIRYFPT

  • 阳性对照
    • RPMI-8826 lysate

性能

应用

Our Abpromise guarantee covers the use of ab102032 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 33 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P 1/100.
ICC/IF 1/100.

靶标

  • 功能
    Catalyzes the exchange of ADP and ATP across the mitochondrial inner membrane.
  • 疾病相关
    Defects in SLC25A4 are a cause of progressive external ophthalmoplegia with mitochondrial DNA deletions autosomal dominant type 2 (PEOA2) [MIM:609283]. Progressive external ophthalmoplegia is characterized by progressive weakness of ocular muscles and levator muscle of the upper eyelid. In a minority of cases, it is associated with skeletal myopathy, which predominantly involves axial or proximal muscles and which causes abnormal fatigability and even permanent muscle weakness. Ragged-red fibers and atrophy are found on muscle biopsy. A large proportion of chronic ophthalmoplegias are associated with other symptoms, leading to a multisystemic pattern of this disease. Additional symptoms are variable, and may include cataracts, hearing loss, sensory axonal neuropathy, ataxia, depression, hypogonadism, and parkinsonism.
  • 序列相似性
    Belongs to the mitochondrial carrier family.
    Contains 3 Solcar repeats.
  • 细胞定位
    Mitochondrion inner membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AAC1 antibody
    • Adenine nucleotide translocator 1 (skeletal muscle) antibody
    • Adenine nucleotide translocator 1 antibody
    • ADP antibody
    • ADP ATP carrier protein 1 antibody
    • ADP ATP carrier protein heart/skeletal muscle isoform T1 antibody
    • ADP/ATP translocase 1 antibody
    • ADT1_HUMAN antibody
    • ANT 1 antibody
    • ANT antibody
    • ANT1 antibody
    • ATP carrier protein 1 antibody
    • ATP carrier protein antibody
    • heart/skeletal muscle isoform T1 antibody
    • PEO2 antibody
    • PEO3 antibody
    • SLC25A4 antibody
    • Solute carrier family 25 member 4 antibody
    • T1 antibody
    see all

Anti-Adenine Nucleotide Translocase 1 antibody 图像

  • Anti-Adenine Nucleotide Translocase 1 antibody (ab102032) at 1 µg/ml + RPMI-8226 Cell Lysate at 10 µg

    Predicted band size : 33 kDa
    12% SDS PAGE
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pineal tissue labelling Adenine Nucleotide Translocase 1 with ab102032 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the cytoplasm of cell bodies of pinealocytes and their processes. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - Adenine Nucleotide Translocase 1. Right - Merge.
  • ab102032 staining Adenine Nucleotide Translocase 1 from  HEK293 cell line from Human by ICC/IF (Immunocytochemistry/immunofluorescence).Cells were  fixed with paraformaldehyde,permeabilized with 1% Triton X-100.Samples were incubated with primary antibody for 16 hours at 4 °C.A diluted Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.

Anti-Adenine Nucleotide Translocase 1 antibody (ab102032)参考文献

ab102032 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Sample
Mouse Cell (MEF)
Specification
MEF
Permeabilization
Yes - 1% TRITON-X-100
Fixative
Paraformaldehyde
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提交于 Sep 09 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Sample
Human Cell (HEK293)
Specification
HEK293
Permeabilization
Yes - 1% TRITON-X-100
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Sep 08 2014

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing
Sample
Rat Cell lysate - whole cell (h9c2)
Specification
h9c2
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Sep 08 2014

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (MEF)
Specification
MEF
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Aug 27 2014

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (HEK293)
Specification
HEK293
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Aug 27 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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