概述

  • 产品名称Anti-Adducin 2抗体
    参阅全部 Adducin 2 一抗
  • 描述
    兔多克隆抗体to Adducin 2
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 648-697 (VVNGREEEQT AEEILSKGLS QMTTSADTDV DTSKDKTESV TSGPMSPEGS) of Human Adducin 2 (NP_001608).

  • 阳性对照
    • Transfected 293T cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab84736 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 81 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin. Calmodulin binds preferentially to the beta subunit.
  • 组织特异性Expressed mainly in brain, spleen, kidney cortex and medulla, and heart. Also expressed in human umbilical vein endothelial cells, human vascular smooth muscle cells, kidney tubular cells and K562 cells.
  • 序列相似性Belongs to the aldolase class II family. Adducin subfamily.
  • 发展阶段Fetal kidney expresses isoforms 3, 4, 5, 6 and 7, and fetal liver expresses isoforms 3 and 4.
  • 结构域Each subunit is comprised of three regions: a NH2-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.
  • 翻译后修饰The N-terminus is blocked.
  • 细胞定位Cytoplasm > cytoskeleton. Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ADD 2 antibody
    • ADD2 antibody
    • ADDB antibody
    • ADDB_HUMAN antibody
    • Adducin 2 (beta) antibody
    • Adducin2 antibody
    • Beta adducin antibody
    • Beta-adducin antibody
    • Erythrocyte adducin subunit beta antibody
    • OTTHUMP00000203378 antibody
    • OTTHUMP00000203908 antibody
    see all

Anti-Adducin 2 antibody 图像

  • Immunocytochemistry/immunofluorescence analysis of Mouse HEI-OCI auditory cells labeling Adducin 2 with ab84736 at 1:100.
  • Anti-Adducin 2 antibody (ab84736) at 1 µg/ml (in 5% skim milk / PBS buffer) + transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 81 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.
    Gel concentration: 12%

Anti-Adducin 2 antibody (ab84736)参考文献

ab84736 has not yet been referenced specifically in any publications.

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