Synthetic peptide derived from the N terminal region of Human ADCY9.
Human lung carcinoma tissue; extracts from HuvEc cells
This antibody gave a positive result when used in the following methanol fixed cell lines: A549.
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.02% Sodium azide Constituents: PBS, 0.88% Sodium chloride, 50% Glycerol
Concentration information loading...
Immunogen affinity purified
ab110159 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml.
1/500 - 1/1000. Predicted molecular weight: 151 kDa.
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
May play a fundamental role in situations where fine interplay between intracellular calcium and cAMP determines the cellular function. May be a physiologically relevant docking site for calcineurin.
Expressed in multiple cells of the lung, with expression highest in airway smooth muscle.
Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.
Contains 2 guanylate cyclase domains.
Information by UniProt
ADCY 9 antibody
Anti-ADCY9 antibody 图像
Immunocytochemistry/ Immunofluorescence - Anti-ADCY9 antibody (ab110159)
ICC/IF image of ab110159 stained A549 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab110159 at 5µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (
ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ADCY9 antibody (ab110159)
ab110159, at a 1/50 dilution, staining ADCY9 in paraffin embedded Human brain tissue by Immunohistochemistry, in the presence (right panel) or absence (left panel) of the immunizing peptide.
Western blot - ADCY9 antibody (ab110159)
All lanes : Anti-ADCY9 antibody (ab110159) at 1/500 dilution Lane 1 : Extracts from HuvEc cells Lane 2 : Extracts from HuvEc cells with immunizing peptide at 10 µg Lysates/proteins at 30 µg per lane. Predicted band size : 151 kDa
Anti-ADCY9 antibody (ab110159)参考文献
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"