概述

  • 产品名称Anti-ADCY9抗体
    参阅全部 ADCY9 一抗
  • 描述
    兔多克隆抗体to ADCY9
  • 经测试应用适用于: ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide derived from the N terminal region of Human ADCY9.

  • 阳性对照
    • Human lung carcinoma tissue; extracts from HuvEc cells This antibody gave a positive result when used in the following methanol fixed cell lines: A549.

性能

应用

Our Abpromise guarantee covers the use of ab110159 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/1000. Predicted molecular weight: 151 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能May play a fundamental role in situations where fine interplay between intracellular calcium and cAMP determines the cellular function. May be a physiologically relevant docking site for calcineurin.
  • 组织特异性Expressed in multiple cells of the lung, with expression highest in airway smooth muscle.
  • 序列相似性Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.
    Contains 2 guanylate cyclase domains.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ADCY 9 antibody
    • ADCY9 antibody
    • ADCY9_HUMAN antibody
    • Adenylate cyclase 9 antibody
    • Adenylate cyclase type 9 antibody
    • Adenylate cyclase type IX antibody
    • Adenylyl cyclase 9 antibody
    • ATP pyrophosphate lyase 9 antibody
    • ATP pyrophosphate-lyase 9 antibody
    • KIAA0520 antibody
    • Type IX adenylyl cyclase antibody
    • Type IX ATP pyrophosphate lyase antibody
    see all

Anti-ADCY9 antibody 图像

  • ICC/IF image of ab110159 stained A549 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab110159 at 5µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • ab110159, at a 1/50 dilution, staining ADCY9 in paraffin embedded Human brain tissue by Immunohistochemistry, in the presence (right panel) or absence (left panel) of the immunizing peptide.
  • All lanes : Anti-ADCY9 antibody (ab110159) at 1/500 dilution

    Lane 1 : Extracts from HuvEc cells
    Lane 2 : Extracts from HuvEc cells with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 151 kDa

Anti-ADCY9 antibody (ab110159)参考文献

ab110159 has not yet been referenced specifically in any publications.

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