概述

  • 产品名称Anti-ADCY7抗体
    参阅全部 ADCY7 一抗
  • 描述
    兔多克隆抗体to ADCY7
  • 经测试应用适用于: IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthesized peptide derived from an internal region of Human ADCY7.

  • 阳性对照
    • NIH3T3 cells; Human brain tissue.

性能

应用

Our Abpromise guarantee covers the use of ab111244 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/500.

靶标

  • 相关性The membrane-bound adenylyl cyclases (ACs) represent one of the major families of effector enzymes for G protein-coupled receptors (GPCRs). Using the high inter-species homology of mammalian AC isoforms, nine Adenylyl cyclase (AC) isoforms, encoded by separate genes, have been identified until today. Human adenylate cyclase genes comprise of 11 to 26 exons, which are distributed over 16 to 430kb. The expression profile of these 9 AC isoforms in a panel of 16 human tissues and in human embryonic kidney (HEK) cells have been demonstrated earlier. The cAMP synthesizing enzymes are found in two forms: cytosolic (soluble) and membrane-bound (particulate). Stimulation of adenylate cyclases produce cAMP form ATP in response to the activation of GPCRs by various hormones, neurotransmitters and other regulatory molecules. cAMP, in subsequent steps down the signal transduction pathway, can stimulate cAMP-dependent protein kinase A (cPKA), and several other target molecules. Activation of cPKA can phosphorylate a broad range of substrates that regulate various metabolic pathways, gene expression, and affect memory functions etc. The stimulation of adenylate cyclases starts with interactions with GPCRs mediated signals initiated by Gs and Gi heterotrimeric G-proteins. The interaction of GPCR agonist (eg. Interaction of isopreternol to beta2 receptors) catalyses the exchange of GDP to GTP that is bound to G proteins. The GTP binding reduces the affinity of GsƒÑ to other GTP binding proteins and Gs-GTP complex stimulate the adenylate cyclase. In last several years, new members of particulate and soluble adenylate cyclase family have been identified and significant progress is made in understanding of the molecular mechanisms that underlie the regulation of these families of enzymes.
  • 细胞定位Integral membrane protein.
  • 数据库链接
  • 别名
    • adenylate cyclase 7 antibody
    • adenylyl cyclase antibody
    • ATP pyrophosphate-lyase antibody
    • KIAA0037 antibody

Anti-ADCY7 antibody 图像

  • Immunohistochemistry analysis of paraffin-embedded Human brain tissue, using 1/50 ab111244. The picture on the right is treated with the synthesized peptid
  • Immunofluorescence analysis of NIH-3T3 cells, using 1/100 ab111244. The picture on the right is treated with the synthesized peptide.

Anti-ADCY7 antibody (ab111244)参考文献

ab111244 has not yet been referenced specifically in any publications.

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