概述

  • 产品名称Anti-ADCY10抗体
    参阅全部 ADCY10 一抗
  • 描述
    兔多克隆抗体to ADCY10
  • 经测试应用适用于: IHC-Fr, WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within amino acids 396-445 (VGHTVRHEYT VIGQKVNLAA RMMMYYPGIV TCDSVTYNGS NLPAYFFKEL) of human ADCY10 (NP_060887).

  • 阳性对照
    • Human fetal brain lysate

性能

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应用

Our Abpromise guarantee covers the use of ab82854 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration. PubMed: 22649251
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 187 kDa.Can be blocked with ADCY10 peptide (ab211578). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

靶标

  • 相关性ADCY10 belongs to a distinct class of mammalian adenylyl cyclase that is soluble and insensitive to G protein or forskolin regulation. It is localized in the cytoplasm and is thought to function as a general bicarbonate sensor throughout the body. It may also play an important role in the generation of cAMP in spermatozoa, implying possible roles in sperm maturation through the epididymis, capacitation, hypermotility, and/or the acrosome reaction.
  • 细胞定位Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton. Cytoplasm, perinuclear region. Note: Distributed to subcellular compartments containing cAMP targets. Found as a plasma membrane associated protein, protein concentrated in the perinuclear region and protein colocalized with actin or tubulin.
  • 数据库链接
  • 别名
    • 3',5'-cyclic AMP synthetase antibody
    • Adenylate cyclase 10 (soluble) antibody
    • Adenylate cyclase 10 antibody
    • Adenylate cyclase homolog antibody
    • AH related protein antibody
    • ATP pyrophosphate-lyase antibody
    • Germ cell soluble adenylyl cyclase antibody
    • HCA2 antibody
    • hsAC antibody
    • hypercalciuria absorptive antibody
    • SAC antibody
    • SACI antibody
    • Sacy antibody
    • Soluble adenylyl cyclase antibody
    • testicular soluble adenylyl cyclase (SAC) antibody
    • Testicular soluble adenylyl cyclase antibody
    see all

Anti-ADCY10 antibody 图像

  • Anti-ADCY10 antibody (ab82854) at 1 µg/ml (in 5% skim milk / PBS buffer) + Fetal Brain lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 187 kDa
    Observed band size : 145 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.

Anti-ADCY10 antibody (ab82854)参考文献

This product has been referenced in:
  • Corredor RG  et al. Soluble adenylyl cyclase activity is necessary for retinal ganglion cell survival and axon growth. J Neurosci 32:7734-44 (2012). IHC-Fr ; Rat . Read more (PubMed: 22649251) »

See 1 Publication for this product

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1.) I suggest to use 10-30ug of total protein per well on the gel. This might have to be optimised, but I recommend to start with 20ug and optimise form the...

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