Anti-ADCK3抗体(ab230897)
Key features and details
- Rabbit polyclonal to ADCK3
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-ADCK3抗体 -
描述
兔多克隆抗体to ADCK3 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow -
免疫原
Synthetic peptide corresponding to Human ADCK3 (internal sequence) conjugated to keyhole limpet haemocyanin.
Database link: Q8NI60 -
阳性对照
- WB: HeLa and U-251 MG whole cell lysates. IHC-P: Human thymus tissue. ICC/IF: HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.01% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab230897于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/100.
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WB |
1/500 - 1/1000. Predicted molecular weight: 72 kDa.
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ICC/IF |
1/100 - 1/500.
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说明 |
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IHC-P
1/100. |
WB
1/500 - 1/1000. Predicted molecular weight: 72 kDa. |
ICC/IF
1/100 - 1/500. |
靶标
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功能
May be a chaperone-like protein essential for the proper conformation and functioning of protein complexes in the respiratory chain. -
组织特异性
Ubiquitously expressed with a relatively greater abundance in heart and skeletal muscle. -
疾病相关
Defects in ADCK3 are a cause of coenzyme Q10 deficiency (CoQ10 deficiency) [MIM:607426]. CoQ10 deficiency is an autosomal recessive disorder with variable manifestations. It can be associated with three main clinical phenotypes: a predominantly myopathic form with central nervous system involvement, an infantile encephalomyopathy with renal dysfunction and an ataxic form with cerebellar atrophy.
Defects in ADCK3 are the cause of spinocerebellar ataxia autosomal recessive type 9 (SCAR9) [MIM:612016]; also known as autosomal recessive cerebellar ataxia type 2 (ARCA2). Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCAR9 is an autosomal recessive form characterized by gait ataxia and cerebellar atrophy with slow progression and few associated features. Patients can manifest brisk tendon reflexes and Hoffmann sign, mild psychomotor retardation, mild axonal degeneration of the sural nerve, exercise intolerance and elevated serum lactate. -
序列相似性
Belongs to the protein kinase superfamily. ADCK protein kinase family.
Contains 1 protein kinase domain. -
细胞定位
Mitochondrion. - Information by UniProt
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数据库链接
- Entrez Gene: 536925 Cow
- Entrez Gene: 56997 Human
- Entrez Gene: 67426 Mouse
- Entrez Gene: 360887 Rat
- Omim: 606980 Human
- SwissProt: Q29RI0 Cow
- SwissProt: Q8NI60 Human
- SwissProt: Q60936 Mouse
see all -
别名
- aarF domain containing protein kinase 3 antibody
- aarF domain-containing protein kinase 3 antibody
- ADCK 3 antibody
see all
图片
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All lanes : Anti-ADCK3 antibody (ab230897) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : U-251 MG (Human brain glioma cell line) whole cell lysate
Predicted band size: 72 kDa -
Formalin-fixed, paraffin-embedded human thymus tissue stained for ADCK3 using ab230897 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ADCK3 (red) using ab230897 at 1/100 dilution followed by DyLight 594-congugated secondary antibody in ICC/IF.
The nuclear counter stain is DAPI (blue).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab230897 被引用在 1 文献中.
- Zhuo BB et al. ADCK1 is a potential therapeutic target of osteosarcoma. Cell Death Dis 13:954 (2022). PubMed: 36371387