概述

  • 产品名称
  • 描述
    兔多克隆抗体to ADAT1
  • 经测试应用
    适用于: WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Dog
  • 免疫原

    Synthetic peptide: AISWSAVPEQ PLDVTANGFP QGTTKKTIGS LQARSQISKV ELFRSFQKLL, corresponding to amino acids 397-446 of Human ADAT1

  • 阳性对照
    • Jurkat cell lysate and Human heart tissue.

性能

应用

Our Abpromise guarantee covers the use of ab50994 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

靶标

图片

  • Lane 1 : MW marker
    Lane 2 : Anti-ADAT1 antibody (ab50994) at 1.25 µg/ml

    Lane 1 : As above
    Lane 2 : Jurkat cell lysate at 10 µg

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 39 kDa
    Observed band size : 39 kDa
  • ab50994 staining of Paraffin embeded Human heart tissue in IHC application at 4-8 ug/ml dilution. Cells with positive label (Myocardial cells) are indicated with arrows. Magnification: 400x

文献

ab50994 has not yet been referenced specifically in any publications.

客户评价及客户问答

There are currently no Customer reviews or Questions for ab50994.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册