Anti-ADAMTSL3抗体(ab104507)

概述

  • 产品名称
    Anti-ADAMTSL3抗体
  • 描述
    兔多克隆抗体to ADAMTSL3
  • 经测试应用
    适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Recombinant fragment, corresponding to amino acids 529-788 of Human ADAMTSL3 (BC128389).

  • 阳性对照
    • Human Fetal Kidney

性能

应用

Our Abpromise guarantee covers the use of ab104507 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/2000. Predicted molecular weight: 189 kDa.
ICC/IF Use at an assay dependent concentration.

靶标

  • 组织特异性
    Expressed in epithelial cells of the colon, fallopian tube, skin, breast, prostate, epididymis, liver, pancreatic islets and bile ducts, as well as by vascular endothelial cells, smooth muscle cells, fibroblasts, cortical and ganglionic neurons and cardiac myocytes. Also expressed by malignant epithelial cells in colon cancer, as well as breast, prostate, renal and skin tumors. Expression is significantly reduced in colon cancer compared to normal colon.
  • 序列相似性
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 PLAC domain.
    Contains 10 TSP type-1 domains.
  • 翻译后修饰
    Glycosylated (By similarity). Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion.
  • 细胞定位
    Secreted, extracellular space, extracellular matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • A disintegrin like and metalloprotease domain with thrombospondin type I motifs like 3 antibody
    • ADAMTS like 3 antibody
    • ADAMTS-like protein 3 antibody
    • ADAMTSL-3 antibody
    • ADAMTSL3 antibody
    • ATL3_HUMAN antibody
    • Punctin-2 antibody
    see all

图片

  • ab104507 staining ADAMTSL3 in Mouse heart valve cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 0.02% serum for 30 minutes at 25°C. Samples were incubated with primary antibody (1/25) for 16 hour at 4°C. An Alexa Fluor®594-conjugated Goat anti-rabbit IgG polyclonal(1/200) was used as the secondary antibody.

    See Abreview

  • Anti-ADAMTSL3 antibody (ab104507) at 1/1000 dilution + Human Fetal Kidney Lysate

    Predicted band size : 189 kDa

文献

ab104507 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Heart Valve)
Specification
Heart Valve
Fixative
Paraformaldehyde
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: .02% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Apr 10 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册