Anti-ADAMTS7抗体(ab28557)

概述

  • 产品名称Anti-ADAMTS7抗体
    参阅全部 ADAMTS7 一抗
  • 描述
    兔多克隆抗体to ADAMTS7
  • 特异性ab28557 recognizes metalloproteinase ADAMTS7.
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to Human ADAMTS7. Immunogen in the spacer-1 region of the C-terminus.

性能

应用

Our Abpromise guarantee covers the use of ab28557 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: 1/1,000 when using colorimetric substrates such as BCIP/NBT and 1/5,000 for chemiluminescent substrates. Predicted molecular weight: 184 kDa. This antibody recognizes the zymogen of ADAMTS7 at around 300 kD in reduced Western blots, activated or alternatively spliced forms at 180-120 kD (major bands) and breakdown products at 58-45 kD in cell lysates. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Metalloprotease that may play a role in the degradation of COMP.
    • 组织特异性Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Detected in meniscus, bone, tendon, cartilage, synovium, fat and ligaments.
    • 序列相似性Contains 1 disintegrin domain.
      Contains 1 peptidase M12B domain.
      Contains 1 PLAC domain.
      Contains 8 TSP type-1 domains.
    • 结构域The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.
      The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • 翻译后修饰N-glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs. N- and C-glycosylations can also facilitate secretion. O-glycosylated proteoglycan. Contains chondroitin sulfate.
      May be cleaved by a furin endopeptidase (By similarity). The precursor is sequentially processed.
    • 细胞定位Secreted, extracellular space, extracellular matrix. Also found associated with the external cell surface.
    • Information by UniProt
    • 数据库链接
    • 别名
      • A disintegrin and metalloprotease with thrombospondin motifs 7 preproprotein antibody
      • A disintegrin and metalloproteinase with thrombospondin motifs 7 antibody
      • A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 7 antibody
      • A disintegrin like and metalloprotease with thrombospondin type 1 motif 7 antibody
      • ADAM metallopeptidase with thrombospondin type 1 motif 7 antibody
      • ADAM metallopeptidase with thrombospondin type 1 motif 7 preproprotein antibody
      • ADAM TS 7 antibody
      • ADAM TS7 antibody
      • ADAM-TS 7 antibody
      • ADAM-TS7 antibody
      • ADAMTS 7 antibody
      • ADAMTS-7 antibody
      • Adamts7 antibody
      • ATS7_HUMAN antibody
      • COMPase antibody
      • DKFZp434H204 antibody
      see all

    Anti-ADAMTS7 antibody (ab28557)参考文献

    ab28557 has not yet been referenced specifically in any publications.

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