Anti-ADAMTS5抗体- Carboxyterminal end (ab39202)

概述

  • 产品名称Anti-ADAMTS5抗体- Carboxyterminal end
    参阅全部 ADAMTS5 一抗
  • 描述
    兔多克隆抗体to ADAMTS5 - Carboxyterminal end
  • 特异性ab39202 recognises the Carboxyterminal end of ADAMTS5.
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide crresponding to aa 850-890 of the carboxyterminal end of human ADAMTS5.

    (Peptide available as ab43061.)

  • 阳性对照
    • IHC-P: Human normal liver parenchym

性能

应用

Our Abpromise guarantee covers the use of ab39202 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 102 kDa. when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. This antibody recognizes the zymogen of ADAMTS5 at 120 kDa in reduced Western blots, activated forms at 73 kDa (major bands), and breakdown products at 50 kDa and 40 kDa in cell lysates. Dilution optimised using Chromogenic detection.

靶标

  • 功能Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. May play a role in proteolytic processing mostly during the peri-implantation period.
  • 组织特异性Expressed at low level in placenta primarily but also detected in heart and brain, cervix, uterus, bladder, esophagus, rib cartilage, chondroblastoma, fibrous tissue and a joint capsule from an arthritic patient.
  • 序列相似性Contains 1 disintegrin domain.
    Contains 1 peptidase M12B domain.
    Contains 2 TSP type-1 domains.
  • 结构域The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • 翻译后修饰The precursor is cleaved by a furin endopeptidase.
  • 细胞定位Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • A disintegrin and metalloproteinase with thrombospondin motifs 11 antibody
    • A disintegrin and metalloproteinase with thrombospondin motifs 5 antibody
    • A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 5 antibody
    • A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 5 (aggrecanase 2) antibody
    • A Disintigrin And Metalloproteinase with ThromboSpondin motif-5 antibody
    • ADAM metallopeptidase with thrombospondin type 1 motif 5 antibody
    • ADAM TS 11 antibody
    • ADAM TS 5 antibody
    • ADAM TS5 antibody
    • ADAM-TS 11 antibody
    • ADAM-TS 5 antibody
    • ADAM-TS5 antibody
    • ADAMTS 11 antibody
    • ADAMTS 5 antibody
    • ADAMTS-11 antibody
    • ADAMTS-5 antibody
    • ADAMTS11 antibody
    • Adamts5 antibody
    • ADMP 2 antibody
    • ADMP-2 antibody
    • ADMP2 antibody
    • Aggrecanase 2 antibody
    • Aggrecanase-2 antibody
    • ATS5_HUMAN antibody
    • FLJ36738 antibody
    • Implantin antibody
    • ThromboSpondin motif-5 antibody
    see all

Anti-ADAMTS5 antibody - Carboxyterminal end 图像

  • Ab39202 staining Human normal liver parenchym. Staining is localized to cytoplasma and secreted.
    Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Anti-ADAMTS5 antibody - Carboxyterminal end (ab39202)参考文献

ab39202 has not yet been referenced specifically in any publications.

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