Anti-ADAMTS4抗体- Carboxyterminal end (ab28285)

概述

  • 产品名称
    Anti-ADAMTS4抗体- Carboxyterminal end
    参阅全部 ADAMTS4 一抗
  • 描述
    兔多克隆抗体to ADAMTS4 - Carboxyterminal end
  • 特异性
    ab28285 recognises the Carboxyterminal end of ADAMTS4. This antibody recognises its zymogen and the activated form.
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human, Rhesus monkey
  • 免疫原

    Synthetic peptide corresponding to the carboxyterminal end of Human ADAMTS4. (Peptide available as ab41235.)

性能

应用

Our Abpromise guarantee covers the use of ab28285 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. 1/1000 when using colorimetric substrates such as BCIP/NBT - 1/5000 when using chemiluminescent substrates. Note: EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen.
IHC-P Use at an assay dependent concentration. PubMed: 21791558

靶标

  • 功能
    Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. Could also be a critical factor in the exacerbation of neurodegeneration in Alzheimer disease. Cleaves aggrecan at the '392-Glu-
    -Ala-393' site.
  • 组织特异性
    Expressed in brain, lung and heart. Expressed at very low level in placenta and skeletal muscles.
  • 序列相似性
    Contains 1 disintegrin domain.
    Contains 1 peptidase M12B domain.
    Contains 1 TSP type-1 domain.
  • 结构域
    The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • 翻译后修饰
    The precursor is cleaved by a furin endopeptidase.
  • 细胞定位
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • A disintegrin and metalloproteinase with thrombospondin motifs 4 antibody
    • A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 4 antibody
    • A disintegrin like and metalloprotease with thrombospondin type 1 motif 4 antibody
    • ADAM metallopeptidase with thrombospondin type 1 motif 4 antibody
    • ADAM TS 4 antibody
    • ADAM TS4 antibody
    • ADAM-TS 4 antibody
    • ADAM-TS4 antibody
    • ADAMTS 2 antibody
    • ADAMTS 4 antibody
    • ADAMTS-4 antibody
    • ADAMTS2 antibody
    • ADAMTS4 antibody
    • ADAMTS4 protein antibody
    • ADMP 1 antibody
    • ADMP-1 antibody
    • ADMP1 antibody
    • Aggrecanase 1 antibody
    • Aggrecanase-1 antibody
    • Aggrecanase1 antibody
    • ATS4_HUMAN antibody
    • KIAA0688 antibody
    see all

Anti-ADAMTS4 antibody - Carboxyterminal end (ab28285)参考文献

This product has been referenced in:
  • Zhou Z  et al. Cerebral cavernous malformations arise from endothelial gain of MEKK3-KLF2/4 signalling. Nature 532:122-6 (2016). WB . Read more (PubMed: 27027284) »
  • Wainwright SD  et al. ADAMTS-4_v1 is a splice variant of ADAMTS-4 that is expressed as a protein in human synovium and cleaves aggrecan at the interglobular domain. Arthritis Rheum 65:2866-75 (2013). WB . Read more (PubMed: 23897278) »

See all 4 Publications for this product

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 27°C
Antigen retrieval step
None
Sample
Mouse Tissue sections (cartilage and meniscus)
Specification
cartilage and meniscus
Permeabilization
No
Fixative
zinc buffered formalin
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提交于 Oct 29 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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