Anti-ADAMTS4抗体(ab185722)
Key features and details
- Rabbit polyclonal to ADAMTS4
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-ADAMTS4抗体
参阅全部 ADAMTS4 一抗 -
描述
兔多克隆抗体to ADAMTS4 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Cow, Orangutan -
免疫原
Recombinant fragment corresponding to Human ADAMTS4 aa 50-350.
Database link: O75173 -
阳性对照
- WB: SH-SY5Y, A-549, SKOV3, 293T cell lysates, mouse and brain tissue lysates. ICC/ IF: C6 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab185722于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (2) |
1/500 - 1/2000. Predicted molecular weight: 90 kDa.
|
ICC/IF | (1) |
1/50 - 1/200.
|
说明 |
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WB
1/500 - 1/2000. Predicted molecular weight: 90 kDa. |
ICC/IF
1/50 - 1/200. |
靶标
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功能
Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. Could also be a critical factor in the exacerbation of neurodegeneration in Alzheimer disease. Cleaves aggrecan at the '392-Glu-
-Ala-393' site. -
组织特异性
Expressed in brain, lung and heart. Expressed at very low level in placenta and skeletal muscles. -
序列相似性
Contains 1 disintegrin domain.
Contains 1 peptidase M12B domain.
Contains 1 TSP type-1 domain. -
结构域
The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
翻译后修饰
The precursor is cleaved by a furin endopeptidase. -
细胞定位
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 286806 Cow
- Entrez Gene: 9507 Human
- Entrez Gene: 240913 Mouse
- Entrez Gene: 100461178 Orangutan
- Entrez Gene: 66015 Rat
- Omim: 603876 Human
- SwissProt: Q9TT93 Cow
- SwissProt: O75173 Human
see all -
别名
- A disintegrin and metalloproteinase with thrombospondin motifs 4 antibody
- A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 4 antibody
- A disintegrin like and metalloprotease with thrombospondin type 1 motif 4 antibody
see all
图片
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Immunofluorescence staining of C6 cells stained for ADAMTS4 with ab185722 at 1/100 dilution. Nuclei are labeled with DAPI (Blue).
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All lanes : Anti-ADAMTS4 antibody (ab185722) at 1/3000 dilution
Lane 1 : SH-SY5Y cell lysate
Lane 2 : A-549 cell lysate
Lane 3 : SKOV3 cell lysate
Lane 4 : 293T cell lysate
Lane 5 : Mouse brain tissue lysate
Lane 6 : Rat brain tissue lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP Goat Anti-Rabbit IgG (H+L)
Developed using the ECL technique.
Predicted band size: 90 kDa
Exposure time: 30 secondsBlocking buffer: 3% nonfat dry milk in TBST.
数据表及文件
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SDS download
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Datasheet download
文献 (59)
ab185722 被引用在 59 文献中.
- Kaufmann JO et al. ADAMTS4-specific MR probe to assess aortic aneurysms in vivo using synthetic peptide libraries. Nat Commun 13:2867 (2022). PubMed: 35606349
- Abu El-Asrar AM et al. Differential Expression and Localization of ADAMTS Proteinases in Proliferative Diabetic Retinopathy. Molecules 27:N/A (2022). PubMed: 36144730
- Zhang J et al. Knockdown of triggering receptor expressed on myeloid cells 1 (TREM1) inhibits endoplasmic reticulum stress and reduces extracellular matrix degradation and the apoptosis of human nucleus pulposus cells. Exp Ther Med 24:607 (2022). PubMed: 36160910
- Zheng X et al. Selumetinib - a potential small molecule inhibitor for osteoarthritis treatment. Front Pharmacol 13:938133 (2022). PubMed: 36238555
- Kement D et al. Neuroserpin Is Strongly Expressed in the Developing and Adult Mouse Neocortex but Its Absence Does Not Perturb Cortical Lamination and Synaptic Proteome. Front Neuroanat 15:627896 (2021). PubMed: 33708076