概述

  • 产品名称Anti-ADAMTS2抗体
    参阅全部 ADAMTS2 一抗
  • 描述
    兔多克隆抗体to ADAMTS2
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide corresponding to the C-terminal sequence of Human ADAMTS2. The sequence differs from the related rat and mouse sequences by three amino acids.

  • 阳性对照
    • Rat brain tissue lysate, Rat heart tissue lysate, Rat cardiac muscle tissue. IF/ICC: PANC-1 cell line

性能

应用

Our Abpromise guarantee covers the use of ab125226 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 135 kDa. The detection limit is approximately 1ng/lane under non-reducing and reducing conditions.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use a concentration of 10 µg/ml.

靶标

  • 功能Cleaves the propeptides of type I and II collagen prior to fibril assembly. Does not act on type III collagen. May also play a role in development that is independent of its role in collagen biosynthesis.
  • 组织特异性Expressed at high level in skin, bone, tendon and aorta and at low levels in thymus and brain.
  • 疾病相关Defects in ADAMTS2 are the cause of Ehlers-Danlos syndrome type 7C (EDS7C) [MIM:225410]. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS7C is marked by extremely fragile tissues, hyperextensible skin and easy bruising. Facial skin contains numerous folds, as in the cutis laxa syndrome.
  • 序列相似性Contains 1 disintegrin domain.
    Contains 1 peptidase M12B domain.
    Contains 1 PLAC domain.
    Contains 4 TSP type-1 domains.
  • 结构域The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.
  • 翻译后修饰The precursor is cleaved by a furin endopeptidase.
  • 细胞定位Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • A disintegrin and metalloproteinase with thrombospondin motifs 2 antibody
    • A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 2 antibody
    • ADAM metallopeptidase with thrombospondin type 1 motif 2 antibody
    • ADAM TS 2 antibody
    • ADAM TS2 antibody
    • ADAM-TS 2 antibody
    • ADAM-TS2 antibody
    • ADAMTS 3 antibody
    • ADAMTS-2 antibody
    • ADAMTS2 antibody
    • ATS2_HUMAN antibody
    • EC 3.4.24.14 antibody
    • EDS VIIB antibody
    • EDS VIIC antibody
    • hPCPNI antibody
    • NPI antibody
    • PC I NP antibody
    • PC I-NP antibody
    • PCINP antibody
    • PCPNI antibody
    • pNPI antibody
    • Procollagen I N proteinase antibody
    • Procollagen I N-proteinase antibody
    • Procollagen I/II amino propeptide processing enzyme antibody
    • Procollagen I/II amino propeptide-processing enzyme antibody
    • Procollagen N endopeptidase antibody
    • Procollagen N-endopeptidase antibody
    see all

Anti-ADAMTS2 antibody 图像

  • ICC/IF image of ab125226 stained PANC-1 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab125226, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab125226, at 1µg/mL, staining paraffin embedded Rat cardiac muscle tissue by immunohistochemistry (IHC-P).
  • All lanes : Anti-ADAMTS2 antibody (ab125226) at 1 µg/ml

    Lane 1 : Rat brain tissue lysate
    Lane 2 : Rat heart tissue lysate


    Predicted band size : 135 kDa

Anti-ADAMTS2 antibody (ab125226)参考文献

This product has been referenced in:
  • Olderøy MØ  et al. Biochemical and structural characterization of neocartilage formed by mesenchymal stem cells in alginate hydrogels. PLoS One 9:e91662 (2014). IHC-P ; Human . Read more (PubMed: 24626259) »

See 1 Publication for this product

Product Wall

Application Western blot
Sample Mouse Tissue lysate - whole (Brain)
Gel Running Conditions Reduced Denaturing
Loading amount 20 µg
Specification Brain
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Jun 06 2016

Abreviews
Application Western blot
Loading amount 45 µg
Gel Running Conditions Reduced Denaturing (Hand-cast gel (4% stacking section and 12% running section))
Sample Mouse Tissue lysate - other (Rat quad, mouse skeletal muscle, HOJ 18¹C)
Specification Rat quad, mouse skeletal muscle, HOJ 18¹C
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Nov 14 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Sample Human Cell (bone cells, Saos2)
Specification bone cells, Saos2
Permeabilization Yes - 0.3 % Triton-X-100
Fixative Paraformaldehyde
Username

Prof. Feray Kockar

Verified customer

提交于 Aug 15 2013

Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody. It is regrettable the results have not been succesful and I fully understand your disappointment.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"